Start-up of high-performance CANON reactor by alternating continuous flow and sequencing batch operation modes

doi:10.11949/j.issn.0438-1157.20170894

CIESC Journal ›› 2018, Vol. 69 ›› Issue (4): 1695-1702.DOI: 10.11949/j.issn.0438-1157.20170894

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Start-up of high-performance CANON reactor by alternating continuous flow and sequencing batch operation modes

CHEN Xi¹, WANG Jianfang^1,2,3, QIAN Feiyue^1,2,3, GAO Junjun¹, SHEN Yaoliang^1,2,3, ZHOU Feng¹

1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, China;
2. National and Local Joint Engineering Laboratory of Resource Utilization Technology of Municipal Sewage, Suzhou 215009, Jiangsu, China;
3. Jiangsu High Education Collaborative Innovation Center of Water Treatment Technology and Material, Suzhou 215009, Jiangsu, China

Received:2017-07-12 Revised:2017-10-13 Online:2018-04-05 Published:2018-04-05
Supported by:
supported by the National Natural Science Foundation of China (51308367, 51578353), the Preponderant Discipline Construction Project in Higher Education of Jiangsu Province, the Scientific Research Innovation Project for Graduate Student of Jiangsu Province (SJLX16-0560) and the Suzhou University of Science and Technology Scientific Research Innovation Project for Graduate Student (SKCX16-032, SKCX16-022).

连续流与序批式组合运行启动高性能CANON反应器

陈希¹, 王建芳^1,2,3, 钱飞跃^1,2,3, 高军军¹, 沈耀良^1,2,3, 周丰¹

1. 苏州科技大学环境科学与工程学院, 江苏苏州 215009;
2. 城市生活污水资源化利用技术国家地方联合工程实验室, 江苏苏州 215009;
3. 江苏高校水处理技术与材料协同创新中心, 江苏苏州 215009

通讯作者: 钱飞跃
基金资助:
国家自然科学基金项目（51308367，51578353）；江苏高校优势学科建设工程资助项目；江苏省研究生科研创新计划项目（SJLX16-0560）；苏州科技大学研究生科研创新计划项目（SKCX16-032，SKCX16-022）。

Abstract

Abstract:

In order to start up a completely autotrophic nitrogen removal over nitrite (CANON) process, the continuous flow and sequencing batch modes were used in stages to operate the completely mixed flow reactor inoculated with nitrosation granular sludges. The variations of reactor performance, sludge morphology and activity, and the microbial community structure were investigated in details. Results showed that sequencing batch operation could promote the increases in both sludge concentration and reaction activity at high influent ammonium loading rate and high dissolved oxygen level, on basis of an appropriate nitrogen substrate ratio obtained in the initial continuous flow stage. Then a superior total nitrogen removal rate of 1.75 kg·(m³·d)^-1 was achieved in the subsequent continuous flow mode. During the operation period, the compactness and settling property of granules were improved obviously. And Miseq high-throughput pyrosequencing analysis revealed that CANON granular sludge showed a relative higher microbial diversity. Corresponding to the specific nitrogen removal rate of 0.24 g·(g VSS·d)^-1, the abundance ratio between aerobic and anaerobic ammonium oxidation bacteria was around 3 to 1, in terms of gene Nitrosomonas and Candidatus Kuenenia, respectively. In addition, few oligotrophic nitrite oxidation bacteria in the granules would not affect the efficiency of CANON process significantly.

Key words: waste water, CANON process, immobilization, continuous flow mode, sequencing batch mode, aerobic, microbial community structure

摘要：

在完全混合流反应器中接种亚硝化颗粒污泥，通过分阶段使用连续流和序批式运行方式，成功启动了全自养生物脱氮（CANON）工艺，并对反应器性能、污泥形态与活性、微生物菌群结构的变化规律进行了深入分析。结果表明，基于初始连续流运行获得的良好基质比，序批式阶段的高氨氮负荷和高溶解氧条件可有效促进污泥浓度与活性的增长，使得反应器在最终连续流状态下的总氮去除负荷达到了1.75 kg·（m³·d）^-1。运行期间，颗粒污泥的密实度和沉降性能均得到改善。由Miseq高通量测序的结果可知，CANON颗粒污泥具有相对较高的微生物多样性。对应于总氮比去除速率0.24 g·（g VSS·d）^-1，Nitrosomonas（好氧氨氧化菌）与Candidatus Kuenenia（厌氧氨氧化菌）丰度比值约为3：1。少量贫营养型亚硝酸盐氧化菌对CANON工艺没有显著影响。

关键词: 废水, CANON工艺, 固定化, 连续流, 序批式, 曝气, 菌群结构

CLC Number:

X703.1

CHEN Xi, WANG Jianfang, QIAN Feiyue, GAO Junjun, SHEN Yaoliang, ZHOU Feng. Start-up of high-performance CANON reactor by alternating continuous flow and sequencing batch operation modes[J]. CIESC Journal, 2018, 69(4): 1695-1702.

陈希, 王建芳, 钱飞跃, 高军军, 沈耀良, 周丰. 连续流与序批式组合运行启动高性能CANON反应器[J]. 化工学报, 2018, 69(4): 1695-1702.

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Start-up of high-performance CANON reactor by alternating continuous flow and sequencing batch operation modes

连续流与序批式组合运行启动高性能CANON反应器

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