CIESC Journal ›› 2023, Vol. 74 ›› Issue (8): 3472-3484.DOI: 10.11949/0438-1157.20230348

• Biochemical engineering and technology • Previous Articles     Next Articles

Immobilization of carbonic anhydrase on modified polyethylene membrane and silica

Lingding MENG(), Ruqing CHONG, Feixue SUN, Zihui MENG, Wenfang LIU()   

  1. School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488, China
  • Received:2023-04-10 Revised:2023-06-22 Online:2023-10-18 Published:2023-08-25
  • Contact: Wenfang LIU


孟令玎(), 崇汝青, 孙菲雪, 孟子晖, 刘文芳()   

  1. 北京理工大学化学与化工学院,北京 102488
  • 通讯作者: 刘文芳
  • 作者简介:孟令玎(1998—),女,硕士研究生,


The activity of carbonic anhydrase (CA) immobilized by different methods was investigated using modified polyethylene (PE) membrane and silica (SiO2) as carriers, and then modified with polydopamine/polyethyleneimine (PDA/PEI) PE- and SiO2-immobilized CA were used as research objects. The optimum reaction conditions and stability were investigated. The results show that under the same reaction conditions, the activity recovery of PDA/PEI-SiO2 immobilized CA was the highest, which was 58.8%, and the activity recovery of PDA/PEI-PE immobilized CA was 17.1%. Their retentive activities were 84.8% and 90.2%, respectively, after 10 use recycles. The optimum reaction conditions of immobilized enzymes were 35℃ and pH 8.5, which were the same as those of free enzymes. The stability of the two immobilized enzymes at higher temperature (55—65℃) and higher acid concentration (>0.010 mol/L) was better than that of free enzyme. Mg2+ could significantly promote the activity of free enzyme and immobilized enzyme, while K+ and Mg2+ had no obvious effect. PDA/PEI-SiO2 and PDA/PEI-PE immobilized CA retained 95.2%和92.4% activity after stored at 4℃ for 10 d. When used in the CO2 hydration reaction, the amount of CaCO3 produced was 120% and 70% of free CA. The immobilized CA has great potential in the industrial application of CO2 capture.

Key words: carbonic anhydrase, immobilization, polyethylene, silica, stability, enzyme activity


分别以改性聚乙烯(PE)膜和氧化硅(SiO2)为载体,考察了不同方法固定化碳酸酐酶(CA)的活性,然后以聚多巴胺/聚乙烯亚胺(PDA/PEI)改性的PE和SiO2固定化CA为研究对象,考察了最适反应条件及稳定性。结果表明:在相同的反应条件下,PDA/PEI-SiO2固定化CA的酶活回收率最高,为58.8%,PDA/PEI-PE固定化CA的酶活回收率为17.1%,使用10次后保留活性分别为84.8%和90.2%;固定化酶的最适反应条件均为35℃、pH 8.5,与游离酶相同;在较高温度(55~65℃)和较高酸浓度(>0.010 mol/L)下的稳定性高于游离酶;Mn2+对游离酶和固定化酶活性均有显著的促进作用,K+、Mg2+无明显影响;PDA/PEI-SiO2和PDA/PEI-PE固定化CA在4℃下贮存10 d,保留活性分别为95.2%和92.4%;当用于CO2水合反应时,CaCO3的生成量分别是游离CA的120%和70%。固定化CA在CO2捕集的工业化应用方面具有很大潜力。

关键词: 碳酸酐酶, 固定化, 聚乙烯, 氧化硅, 稳定性, 酶活

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