Abstract: An integrated bioprocess of cellulase-catalysed conversion of steroidal saponins and extration of diosgenin was investigated by using a three-liquid-phase system. The distribution of several steroidal saponins, glucose, enzyme，and its activity was studied in a three-liquid-phase system composed of n-hexane, 1,4-dioxane and ammonium sulfate. And the yield of diosgenin obtained in the three-liquid-phase system was compared with that in organic phase and aqueous phase. The experimental results showed that the three-liquid-phase system could be satisfied for simultaneous enzymatic hydrolysis of steroidal saponins and extration of diosgenin and glucose. The substrate and enzyme were held in the middle phase of 1,4-dioxane, whiles the products, diosgenin and glucose, were partitioned into the top and bottom phase, respectively. The yields of diosgenin was 69.4% at 96 h in the three-liquid-phase system，which was twice and 27.6 folds obtained in organic phase and aqueous phase respectively.

Key words: diosgenin；threeliquidphase extraction, enzymatic hydrolysis, cellulase

摘要： 利用三液相萃取与酶催化转化耦合技术对盾叶薯蓣中薯蓣皂苷进行水解，并分离提取薯蓣皂苷元。考察了几种甾体皂苷和水解下的葡萄糖在三液相中的分配、酶的分配以及酶活力在三液相体系中的保留情况，并对比了三液相、有机相、水相中纤维素酶催化转化与薯蓣皂苷元的生成情况。实验结果表明，正己烷/1,4-二氧六环/硫酸铵三液相体系能够满足酶催化和产物分离同步进行的要求，使薯蓣皂苷元分配在正己烷相，底物和酶保留在1,4-二氧六环相，大部分葡萄糖进入硫酸铵相，反应进行96h薯蓣皂苷元的得率为69.4%，比有机相、水相酶催化分别提高一倍和27.6倍，显示出耦合技术的明显优势。

关键词: 薯蓣皂苷元, 三液相萃取, 酶催化转化, 纤维素酶