Growth and interaction mechanism of multi-strain biofouling under different nutrient levels

doi:10.3969/j.issn.0438-1157.2013.03.035

CIESC Journal ›› 2013, Vol. 64 ›› Issue (3): 1036-1041.DOI: 10.3969/j.issn.0438-1157.2013.03.035

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Growth and interaction mechanism of multi-strain biofouling under different nutrient levels

YANG Qianpeng¹, CHEN Xiaodong², TIAN Lei¹, SHI Lin¹

1. Key Laboratory for Thermal Science and Power Engineering of Ministry of Education, Department of Thermal Engineering, Tsinghua University, Beijing 100084, China;
2. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China

Received:2012-05-23 Revised:2012-06-27 Online:2013-03-05 Published:2013-03-05
Supported by:
supported by the National Natural Science Foundation of China(50976060)and the National Basic Research Program of China(2010CB227305).

不同营养下混合菌种微生物污垢生长机理与交互作用

杨倩鹏¹, 陈晓东², 田磊¹, 史琳¹

1. 清华大学热能工程系,热科学与动力工程教育部重点实验室,北京 100084;
2. 厦门大学化学化工学院化学工程与生物工程系,福建厦门 361005

通讯作者: 史琳
作者简介:杨倩鹏(1989—),男,博士研究生。
基金资助:
国家自然科学基金项目(50976060);国家重点基础研究发展计划项目(2010CB227305)。

Abstract

Abstract: Heat pump system is an effective method for heat recovery of a treated sewage source,but biofouling on heat exchanger is still a problem to be solved,and may affect seriously efficiency and security of the system.By long term monitoring and 16S rRNA identification,two major bacteria genus,Bacillus sp. and Aeromonas sp.,were selected for multi-strain biofouling experiment of a model fluid in laboratory.By the experiment of foul growth performed on a purpose made channel at various bacteria ratios and nutrient levels,the regular patterns of single and mixed microbe growth of Bacillus sp. and Aeromonas sp. were revealed,their synergy and inhibition interaction summarized,and the effect of nutrient levels on their growth mechanism and interaction also investigated.The results indicated that the Bacillus sp. has strong ability in biofouling,in high multiple rates,and high tolerance to poor nutrient level.The biofouling of Aeromonas sp. is weak relatively,but high secretion rate that may help for the synergy growth with Bacillus sp.Their synergy and inhibition interactions co-exist and dominate in turn under different growth stage and condition.

Key words: biofouling, multi-strain, growth mechanism, synergy and inhibition interactions

摘要： 再生水源热泵能有效回用废热,但再生水换热器的微生物污垢问题一直没有完全解决,严重影响换热效率和系统安全。利用长期监测并且进行16S rRNA鉴定得到的再生水及其污垢中两种主要代表菌属芽孢杆菌属和气单胞菌属,在实验室内配制模拟流体进行混合菌种微生物污垢实验。通过在特制的机理流道上进行不同菌种比例和不同营养物质浓度的污垢生长实验,得出芽孢杆菌属和气单胞菌属的单独生长规律和混合生长规律,总结了混合生长时两种菌属的协同和抑制规律,同时考察了营养物质浓度对生长规律和交互作用的影响。结果表明:芽孢杆菌属成垢能力较强,细胞分裂速度高,耐贫营养能力强;气单胞菌属成垢能力弱于芽孢杆菌属,但分泌物生成速率高,可以与芽孢杆菌属协同生长;两种菌属交互作用在不同阶段和条件下,协同与抑制作用共存并轮流占据主导。

关键词: 微生物污垢, 混合菌种, 生长机理, 协同与抑制

CLC Number:

TK01

YANG Qianpeng, CHEN Xiaodong, TIAN Lei, SHI Lin. Growth and interaction mechanism of multi-strain biofouling under different nutrient levels[J]. CIESC Journal, 2013, 64(3): 1036-1041.

杨倩鹏, 陈晓东, 田磊, 史琳. 不同营养下混合菌种微生物污垢生长机理与交互作用[J]. 化工学报, 2013, 64(3): 1036-1041.

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Growth and interaction mechanism of multi-strain biofouling under different nutrient levels

不同营养下混合菌种微生物污垢生长机理与交互作用

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