Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

doi:10.3969/j.issn.0438-1157.2013.08.038

CIESC Journal ›› 2013, Vol. 64 ›› Issue (8): 2969-2974.DOI: 10.3969/j.issn.0438-1157.2013.08.038

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Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

LI Weijie, ZHOU Xinli, LIU Baolin, LÜ Fukou

Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China

Received:2013-01-31 Revised:2013-03-22 Online:2013-08-05 Published:2013-08-05
Supported by:
supported by the National Natural Science Foundation of China(50906057,51076108)and Shanghai Leading Academic Discipline Project(S30503).

升降温速率对低温保护剂溶液结晶性质的影响

李维杰, 周新丽, 刘宝林, 吕福扣

上海理工大学生物热科学研究所, 上海 200093

通讯作者: 周新丽
作者简介:李维杰(1983- ),男,博士。
基金资助:
国家自然科学基金项目(50906057,51076108);上海市重点学科项目(S30503)。

Abstract

Abstract: When cells are cryopreserved,the morphology of cryoprotectant at crystallization and recrystallization would influence the survival rate of cells.The cryo-microscope system was used to observe the morphology of crystal and sizes of two cryoprotectants during cooling and rewarming at different speeds.It is found that crystals during cooling are smaller and less at higher speed than at lower speed,and the former is favorable for preservation of cells.When the vitrified cryoprotetant is rewarmed,crystals during recrystallization are smaller and less at higher speed than at lower speed,and the former with narrow danger zone is favorable for the survival of oocytes.Besides,high concentration of cryoprotectant is more favorable for the survivalrate of oocytes than lower concentration.

Key words: cryo-microscope, cooling rate, rewarming rate, crystallization, cryopreservation

关键词: 低温显微镜, 降温速率, 升温速率, 结晶, 低温保存

CLC Number:

TK6
Q599

LI Weijie, ZHOU Xinli, LIU Baolin, LÜ Fukou. Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes[J]. CIESC Journal, 2013, 64(8): 2969-2974.

李维杰, 周新丽, 刘宝林, 吕福扣. 升降温速率对低温保护剂溶液结晶性质的影响[J]. 化工学报, 2013, 64(8): 2969-2974.

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Effect of cooling and rewarming rate on crystallization of cryoprotectant solution for oocytes

升降温速率对低温保护剂溶液结晶性质的影响

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