Abstract: Radio-frequency atmospheric pressure glow discharge (RF APGD) plasmas have outstanding features of no need of expensive and complicated vacuum system, low gas temperatures, high concentrations of reactive species, good discharge uniformity, strong controllability and diverse interactions with various biomolecules, which have attracted more and more attentions for their applications in biotechnology. Our research group introduced the RF APGD plasma jet into the mutation breeding field. Based on the study on the physical characteristics of RF APGD plasmas and their interaction mechanisms with bio-macromolecules and whole cells, a novel mutation breeding system, named as atmospheric and room temperature plasma (ARTP) mutation breeding machine was invented. The ARTP mutation breeding system has the features of high safety for the operators and environment, easy operation, rapid mutation capability, high mutation rate and genetic stability of targeted mutants, as demonstrated by successful mutation for more than 40 different microbes including bacteria, fungi and algae. This paper reviews the recent research progress of ARTP mutation breeding technology, hopefully contributing to the development of the life evolution study and the industrial strain modification as a powerful mutation tool.

Key words: radio frequency atmospheric pressure glow discharge, atmospheric and room temperature plasma, mutagenesis, mutation breeding

摘要： 大气压射频辉光放电（RF APGD）等离子体具有大气压下操作不需要真空系统、气体温度低、活性粒子浓度高、放电均匀性好、可控性强等特点，能够与各类生物分子发生作用，在生物技术中的应用受到广泛的关注。本研究团队将RF APGD等离子体射流引入生物诱变育种领域，对其物理特性及其与生物大分子和整细胞的作用机制进行了系统研究，并将其开发成新一代高效诱变育种仪，命名为常压室温等离子体（ARTP）诱变育种仪。实践证明，ARTP诱变育种仪具有对操作者安全、环境友好、操作简便、突变快速、突变率高、获得的突变体性状稳定等特点，目前已成功应用于包括细菌、放线菌、真菌、酵母、微藻等在内的四十余种微生物的诱变育种。本文将对ARTP生物育种技术的最新研究进展进行综述，以期ARTP快速生物突变技术在生物进化研究及工业生物菌种改造上发挥重要作用。

关键词: 大气压射频辉光放电, 常压室温等离子体, 诱变, 生物育种