Abstract: The reaction of immobilized enzyme in spherical porous support coupled with intraparticle and external mass transfer were studied in a batch reactor. An exact solution for the case of the first order reaction was presented. The orthogonal collocation method was used to simulate the complex systems. The catalytic reaction (the Michaelis - Menten equation as example), enzyme inactivation rate and the intraparticle and external mass transfer resistance of the substrate were taken into account to research the distribution of the substrate concentration in porous support, the changes of the bulk concentration and conversion with time.

摘要： <正>多孔载体固定化酶催化反应技术具有许多优点,在工业生产中的应用日益增多。大多数酶在固定化后,由于酶构象改变、分配效应和扩散限制等的影响,反应速率有所下降,其稳定性等亦发生变化。如何测定和定量表征这些性态,对酶催化反应器的设计和操作十分重要。