Abstract: In order to overcome the elementary heterogeneous nucleation while octahydro trisodium salt
of fructose-1,6-diphosphate(FDPNaa.8 H2O) is crystallized with ethanol precipitation at low
temperature, a new crystallizationmethod with alcohol precipitation combined with salt
precipitation has been presented. The ethanol-sodium ac-etate system for crystallization of
salt of fructose-1,6-diphosphate is based on the mechanism of crystallization ofFDPNa3.8
H2O in the ethanol-low temperature system. It is found that crystal size may be controlled
by regulatingtemperature or pH value of solution in the crystallization process, and the
crystal yield increases to 95% from 78%which obtained in the ethanol-low temperature
system.

Key words: octahydro trisodium salt of fructose-1, 6-diphosphate, ethanol-sodium acetate system, crystallization

摘要： In order to overcome the elementary heterogeneous nucleation while octahydro trisodium salt
of fructose-1,6-diphosphate(FDPNaa.8 H2O) is crystallized with ethanol precipitation at low
temperature, a new crystallizationmethod with alcohol precipitation combined with salt
precipitation has been presented. The ethanol-sodium ac-etate system for crystallization of
salt of fructose-1,6-diphosphate is based on the mechanism of crystallization ofFDPNa3.8
H2O in the ethanol-low temperature system. It is found that crystal size may be controlled
by regulatingtemperature or pH value of solution in the crystallization process, and the
crystal yield increases to 95% from 78%which obtained in the ethanol-low temperature
system.

关键词: octahydro trisodium salt of fructose-1;6-diphosphate;ethanol-sodium acetate system; crystallization