Abstract: Lysozyme extraction by mixed reverse micelles composed of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) and tetraethylene glycol phenylnonyl ether (PNE4) was studied. Taking into account the contributions of electrostatic and hydrophobic interactions, a theoretical model was established to describe the partitioning equilibrium. Experimental results demonstrated the reliability and usefulness of the model. It was found that the hydrophobicity of AOT was lower in the presence of Na⁺ than of K⁺, while that of PNE4 was independent of the cations. The enhancement of lysozyme surface hydrophobicity by Na⁺ was more significant than K⁺, while the adsorption ability of K⁺ on the inner surface of the reverse micelles was larger than Na⁺. Analyses and explanations of these phenomena were made by using electrostatic and thermodynamic theories.

Key words:

反胶团萃取, 蛋白质, 分配平衡, 模型

摘要： 研究了二(2-乙基己基)琥珀酸酯磺酸钠(AOT)和四聚氧乙烯基苯壬基酚醚(PNE4)混合表面活性剂反胶团系统萃取溶菌酶的平衡行为，建立了考虑静电作用和疏水性作用贡献的反胶团萃取蛋白质的分配平衡模型，实验验证了模型的可靠性和适用性. 研究发现，有Na⁺存在时AOT的疏水性较K⁺存在时小，而PNE4的疏水性因子与盐离子种类无关；Na⁺对溶菌酶表面疏水性的增大效应较K⁺显著；K⁺在反胶团内界面上的吸附作用大于Na⁺. 利用静电学和热力学理论讨论和解释了有关现象.

关键词:

反胶团萃取, 蛋白质, 分配平衡, 模型