Abstract: Thymosin α₁ was synthesized manually. The washing condition and the test of the degree of coupling were studied. In addition, the analytical method of crude product was set up. Thymosin α₁ was synthesized with the solid-phase method, by using the base-liable Fmoc group to protect the α-amino acid and N,N′-diisopropylcarbodiimide (DIC) as the coupling regent. The crude product was analyzed by reversed-phase high performance liquid chromatography, SDS-PAGE and liquid chromatography/mass spectroscopy.The synthesis yield was 84.89%. The analytical results indicated that the crude product contained much thymosin α₁, and the purity was 50.3%. DIC solid-phase chemical synthesis of thymosin α₁ was applicable, and the yield was improved greatly compared to other known methods.

Key words: 胸腺素α₁, 固相多肽合成, 二异丙基碳二亚胺（DIC）, 反相高效液相色谱法, 聚丙烯酰胺凝胶电泳（SDS-PAGE）法, 液相色谱/质谱（LC/MS）

关键词: 胸腺素α₁, 固相多肽合成, 二异丙基碳二亚胺（DIC）, 反相高效液相色谱法, 聚丙烯酰胺凝胶电泳（SDS-PAGE）法, 液相色谱/质谱（LC/MS）