Abstract:

Ribavirin production by the mixed fermentation of Bacillus substilis TM903 and Corynebacterium glutamicum TL1105 was studied.The inoculum size of TL1105 and the optimal addition of KH2PO4, MnSO4, 1H-[BF]1,2,4-[BFQ]triazole-3-carboxamide (TCA) in the fermentation medium were established initially.Then, the factorial design by MINITAB software was employed to optimize the adding mode of glucose, KH2PO4, MnSO4, TCA, which suggested that MINITAB software was the more effective tool in the culture medium optimization.Under the optimal conditions, the yield of ribavirin was 4.69 g·L-1 after 60 h fermentation with glucose added one-off and KH2PO4, Mn+and TCA fed in batches.

Key words:

混合菌发酵, 三氮唑核苷, MINITAB, 析因实验设计

摘要： 以枯草芽孢杆菌（Bacillus substilis）TM903和谷氨酸棒杆菌（Corynebacterium glutamicum）TL1105为供试菌株，进行混合菌发酵法生产三氮唑核苷的研究。通过对TL1105接种量、KH2PO4、Mn2+以及前体物1H-1,2,4-三氮唑-3-甲酰胺（简称TCA）添加量的研究初步确定三氮唑核苷的发酵工艺条件。采用MINITAB软件对葡萄糖、KH2PO4、Mn2+及TCA的添加方式进行析因实验设计，得出最佳发酵条件，即一次性投糖，KH2PO4、Mn2+及TCA则进行分批流加。在最佳发酵条件下进行三氮唑核苷混合菌发酵，经过发酵60h，发酵液中可积累4.69g•L-1的三氮唑核苷。

关键词:

混合菌发酵, 三氮唑核苷, MINITAB, 析因实验设计