Abstract:

Mixed-mode chromatography is a new kind of chromatography technology for bioseparation.The special ligand combines two or more interaction modes, commonly hydrophobic and electrostatic interactions.Mixed-mode chromatography can directly capture target protein from moderate-ionic-strength feedstock without the need of dilution or other additives, which certainly improves the process efficiency and reduces the cost of primary recovery in the downstream process.This paper reviews the current advances in mixed-mode chromatography, as well as technical history and some applications.The future development and application areas are also discussed.

Key words: 生物分离, 层析, 混合模式, 耐盐吸附, 疏水性电荷诱导层析, 配基结构

摘要：

混合模式吸附层析是一种新型的生物分离方法，其功能配基兼有两种或两种以上的作用模式，主要为静电和疏水相互作用。在低盐和高盐条件下，都可以实现对目标物的有效吸附，具有明显的耐盐吸附的特性，可避免对料液的额外预处理，从而提高分离效率，减少分离成本，已获得一定的应用。本文对混合模式吸附技术的发展历程、类型及应用现状等进行综述，以推动该技术的发展和进一步应用。

关键词: 生物分离, 层析, 混合模式, 耐盐吸附, 疏水性电荷诱导层析, 配基结构