Abstract:

The effect of different surfactants on α-arbutin bio-catalytic synthesis by Xanthomonas maltophilia BT-112 was investigated.Tween80 was the best when compared with different kinds of surfactants at different concentrations and times of surfactant addition.Experimental results showed that the suitable conditions were as follows: adding 3 g·L^-1 Tween80 during the 12 hours from the start of fermentation, fed-batch in three times, 1 g·L^-1 every time.Under the conditions mentioned above, the product yield reached 96.2% against hydroquinone and increased by 3.53% and hydroquinone concentration reached 60 mmol·L^-1 and increased by 25.0% as compared with the case without adding surfactant.The fermentation period was 36 h, 25% less than the case without adding surfactant.

Key words:

α-熊果苷, 黄单胞菌, 表面活性剂

摘要： 探讨了不同表面活性剂对嗜麦芽黄单胞菌BT-112(Xanthomonas mahophilia BT-112) 催化合成α-熊果苷的影响。通过比较不同种类、不同浓度的表面活性剂及其加入时间，实验得出最佳表面活性剂为Tween80，最适反应条件为：Tween80浓度为3 g·L^-1，加入时间为12h，流加次数为3次，每次浓度1 g·L^-1。在此条件下对苯二酚的转化率为96.2 %，菌体对对苯二酚的最大耐受度为60 mmol·L^-1 ,分别比空白提高了3.53%和25.0%，发酵周期为36h，比空白缩短了25%。

关键词:

α-熊果苷, 黄单胞菌, 表面活性剂