Abstract: Bioconversion of L-phenylalanine to 2-phenylethylalcohol with yeasts was performed in an aqueous/organic solvent biphasic system. Saccharomyces cerevisiae CICIM Y0086（T）had more potential to transform L-phenylalanine to 2-phenylethylalcohol than Kluyvermyces marxianus AS.2.1440. The results of the solvent screening indicated that oleic acid was the best solvent which could extract 2-phenylethylalcohol efficiently among the four kinds of solvent. When the concentration of L-phenylalanine was 6 g/L，the optimum values of volume ratio of organic phase to aqueous phase，reaction temperature，pH value and liquid volume were 1∶1，30 ℃，4.0 and 20 mL/250 mL respectively. Under above optimum conditions，the content of 2-phenylethylalcohol reached 3.0 g/L 48 h later，which increased by about 50% than that in monophasic aqueous system.

摘要： 研究了水/有机溶剂两相体系中，酵母细胞转化L-苯丙氨酸（L-phe）生成2-苯乙醇（PEA）的过程。菌种选择表明酿酒酵母CICIM Y0086（T）的转化能力要高于马克斯克鲁维酵母AS.2.1440，有机溶剂的选择表明，在实验使用的4种有机溶剂中，油酸为该反应最适有机溶剂。当初始底物浓度为6 g/L、水和油酸的相体积比为1∶1、反应温度30 ℃和pH值4.0、装液量20 mL/250 mL 的条件下，转化48 h，产物浓度达到3.0 g/L，比单水相提高了50%。