Abstract:

A heterotrophic nitrifier with high efficiency of removing NH₄⁺-N and significant accumulation of NO₂^--N， named strain C16， was isolated from the activated sludge of a cokes wastewater treatment facility .Cells of strain C16 were Gram negative， short rods.The colonies were translucent white.It was identified as Alcaligenes sp.according to its morphological and physiological properties and the analysis of its 16S rRNA gene sequence.Results show that all NH₄⁺-N have been removed.The removal rate of NH₄⁺-N by this strain is 94.7% and the accumulation of NO₂^--N is tremendous in the optimization medium after 4 d cultivation through the experiment of medium optimization.The concentration of NO₂^--N is up to 30.1 mg·L^-1 compared with the counterpart original cultivation which is just 7.34 mg·L^-1.

Key words: FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">异养硝化；分离；培养基优化；产碱杆菌FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>C16

摘要：

采用传统微生物分离纯化方法，从焦化废水活性污泥中筛选到一株高效去除氨氮并显著积累亚硝酸盐氮的异养硝化细菌C16。该菌株为G^-，短杆状；菌落为白色、半透明。经形态、生理生化特性以及16S rRNA基因序列分析，初步鉴定该菌属于产碱杆菌属(命名为Alcaligenes sp.C16)。对该菌的异养硝化功能进行了研究，结果表明，在异养硝化过程中，该菌可将培养基中的氨氮全部去除。采用优化后的培养基，经4 d培养，菌株C16的氨氮去除率为94.7%，亚硝酸盐氮的积累量大幅度增加，在培养基中氨氮浓度同为100 mg·L^-1条件下，由优化前的7.34 mg·L^-1增长到优化后的30.1 mg·L^-1。

关键词: FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Times New Roman, mso-hansi-font-family: Times New Roman, mso-bidi-font-family: Times New Roman, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">异养硝化；分离；培养基优化；产碱杆菌FONT-SIZE: 10.5pt, mso-bidi-font-size: 11.0pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang=EN-US>C16