Abstract: he effect of using sugarcane molasses and whey powder to prepare succinic acid by the fermentation of Actinobacillus succinogenes NJ113 was investigated. When the concentration of glucose was 40 g/L, the production of succinic acid reached to 26.04 g/L, and which was 28.27 g/L when using the same concentration of molasses as carbon source. It indicates that the production of succinic acid using sugarcane molasses was improved by 8.57%. When whey powder and extract yeast was used as nitrogen source, the optimal concentrations of whey powder and extract yeast were 8 g/L and 2 g/L respectively. With 40 g/L molasses, 8 g/L whey powder and 2 g/L yeast powder in a 3 L fermenter, the production of succinic acid was 32.54 g/L and the yield of succinic acid reached to 81.13%.

Key words: sugarcane molasses, whey powder, Actinobacillus succinogenes NJ113, succinic acid

摘要： 考察了甘蔗糖蜜替代昂贵葡萄糖作为碳源、乳清粉替代大部分酵母粉作为氮源时，对Actinobacillus succinogenes NJ113发酵制备丁二酸的影响。血清瓶厌氧发酵结果证明：对照组（葡萄糖40 g/L）的丁二酸产量仅为26.04 g/L，而以糖蜜为碳源（以总还原糖计算为40 g/L）时，丁二酸产量达到28.27 g/L，比对照组提高了8.57%。在此基础上，以糖蜜为碳源、不同比例的乳清粉和酵母粉为混合氮源发酵制备丁二酸，确定了糖蜜、乳清粉和酵母粉混合使用的最佳浓度分别为40 g/L、8 g/L和2 g/L。此外，在3 L发酵罐体系中添加40 g/L糖蜜、8 g/L乳清粉、2 g/L酵母粉进行发酵试验，实验结果证明：丁二酸终产量达到32.54 g/L，收率达到81.13%。

关键词: 甘蔗糖蜜, 乳清粉, Actinobacillus succinogenes NJ113, 丁二酸