Research advances in metabolic control of succinic acid fermentation

doi:10.11949/j.issn.0438-1157.20141332

Abstract

Abstract:

As an important chemical raw material, succinic acid is widely used in many fields of modern industry with a huge market demand. Recently, the biological method for succinic acid fermentation has become a hot topic in research, since it has the advantages of renewable utilization of resources and environmental friendly production. From the perspective of metabolic control, the methods and strategies for succinic acid production with some strains, especially the recombinant E.coli, are summarized in this paper. Regulation of key enzyme gene in metabolism branch and the key factors in fermentation of succinic acid, such as regulation of redox force, carbon dioxide control, and metabolic pathways transforming are introduced. The progress of metabolic control of succinic acid fermentation is presented, and development of succinic acid fermentation in the future is proposed.

Key words: recombinant E.coli, succinic acid, biotechnology, metabolism, fermentation, carbon dioxide

摘要：

琥珀酸作为一种重要的化工原料被广泛应用于现代工业的各个领域,具有庞大的市场需求。利用生物法发酵生产琥珀酸因具有资源可再生利用、绿色清洁节能等方面不可替代的优势而成为当今研究的热点。本文从代谢调控的角度出发分析总结了一些产琥珀酸菌株,尤其是重组大肠杆菌在发酵生产琥珀酸中所应用到的方法和策略,如对代谢途径中关键酶的调控以及发酵生产中的关键因素(如氧化还原回复力调控、CO₂调节、代谢途径改造等),着重突出了代谢控制发酵生产方面的研究进展,并对今后的发展方向提出了一些设想。

关键词: 重组大肠杆菌, 琥珀酸, 生物技术, 代谢, 发酵, 二氧化碳

CLC Number:

Q599

WANG Le, NI Zifu, HUI Ming, WANG Jinshui. Research advances in metabolic control of succinic acid fermentation[J]. CIESC Journal, 2015, 66(4): 1243-1251.

王乐, 倪子富, 惠明, 王金水. 代谢控制发酵产琥珀酸研究进展[J]. 化工学报, 2015, 66(4): 1243-1251.

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