Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC

doi:10.11949/j.issn.0438-1157.20181483

Abstract

Abstract:

Objective to establish a method for the determination of resveratrol and polydatin in peanut bud and grape pip extract. High performance liquid chromatography (HPLC) was used. The chromatographic column was Di Ma C₁₈, the mobile phase was acetonitrile water solution, gradient elution, flow rate 1 ml/min, column temperature 30℃, 306 nm and 20 μl. The linear range of resveratol showed good linear relationship 0.343－15.15×10³μg /ml, R² = 0.9967. The linear range of polydatin showed good linear relationship 110.712－1774.23×10³ μg/ml, R² = 0.9993. The method is rapid, simple and reproducible, and is suitable for quantitative analysis of resveratrol in peanut sprouts and grape seeds.

Key words: microwave ultrasonic wave, extraction, resveratrol, pharmaceuticals, chromatography, leaching

摘要：

建立了花生芽和葡萄籽微波超声波联用提取白黎芦醇及其苷的HPLC测定方法。采用高效液相色谱进行测定,色谱柱为迪马C₁₈, 流动相为乙腈-水溶液，梯度洗脱，流速1.0 ml/min，柱温为30℃，检测波长为306 nm，进样量为20 μl。白黎芦醇在0.343～15.15×10³ μg/ml范围内呈良好的线性关系，相关系数R² = 0.9967；白藜芦醇苷在110.712～1774.23×10³ μg /ml范围内线性关系良好，相关系数R² = 0.9993。该方法检测快速、简便、重现性好，适用于花生芽和葡萄籽中白藜芦醇及其苷的定量分析。

关键词: 微波超声波, 萃取, 白藜芦醇, 药物, 色谱, 浸取

CLC Number:

R 284

Mixia MA, Ning QIN, Qing MIN, Wenxiang HU. Resveratrol and polydatin by microwave-ultrasound extraction and determination by HPLC[J]. CIESC Journal, 2019, 70(S1): 124-129.

马密霞, 秦宁, 闵清, 胡文祥. 微波超声波联用萃取白藜芦醇及其苷的HPLC测定[J]. 化工学报, 2019, 70(S1): 124-129.

Figures/Tables 6

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水平	因素
水平	乙醇浓度(A)/%	提取时间(B)/min	提取温度(C)/℃
1	40	3.0	65
2	50	3.5	70
3	60	4.0	75
4	70	4.5	80

水平	因素
水平	乙醇浓度(A)/%	提取时间(B)/min	提取温度(C)/℃
1	40	3.0	65
2	50	3.5	70
3	60	4.0	75
4	70	4.5	80

实验号	因素			浸膏质量/g	浸膏得率/%	浸膏得率评分	白藜芦醇含量/%	白藜芦醇含量评分	综合评分
实验号	A	B	C	浸膏质量/g	浸膏得率/%	浸膏得率评分	白藜芦醇含量/%	白藜芦醇含量评分	综合评分
1	1	1	1	0.90	30.00	22.64	0.4977	60.00	82.64
2	1	2	2	1.59	53.00	40.00	0.2932	35.35	75.35
3	1	3	3	0.76	25.33	19.12	0.2819	33.98	53.10
4	1	4	4	0.97	32.33	24.40	0.0378	4.56	28.96
5	2	1	2	0.87	29.00	21.89	0.0328	3.95	25.84
6	2	2	1	0.80	26.67	20.13	0.0225	2.71	22.84
7	2	3	4	1.25	41.67	31.45	0.0348	4.20	35.64
8	2	4	3	1.26	42.00	31.70	0.0076	0.92	32.61
9	3	1	3	1.33	44.33	33.46	0.0259	3.12	36.58
10	3	2	4	1.12	37.33	28.18	0.0060	0.72	28.90
11	3	3	1	1.03	34.33	25.91	0.0486	5.86	31.77
12	3	4	2	1.00	33.33	25.16	0.0247	2.98	28.13
13	4	1	4	1.06	35.33	26.67	0.0281	3.39	30.05
14	4	2	3	0.68	22.67	17.11	0.0900	10.85	27.96
15	4	3	2	0.97	32.33	24.40	0.0193	2.33	26.73
16	4	4	1	0.95	31.67	23.90	0.0860	10.37	34.27
K₁	60.01	43.78		42.88
K₂	29.23	38.76		22.29
K₃	31.35	36.81		37.56
K₄	29.75	30.99		30.89
R	0.78	12.79		20.59

实验号	因素			浸膏质量/g	浸膏得率/%	浸膏得率评分	白藜芦醇含量/%	白藜芦醇含量评分	综合评分
实验号	A	B	C	浸膏质量/g	浸膏得率/%	浸膏得率评分	白藜芦醇含量/%	白藜芦醇含量评分	综合评分
1	1	1	1	0.90	30.00	22.64	0.4977	60.00	82.64
2	1	2	2	1.59	53.00	40.00	0.2932	35.35	75.35
3	1	3	3	0.76	25.33	19.12	0.2819	33.98	53.10
4	1	4	4	0.97	32.33	24.40	0.0378	4.56	28.96
5	2	1	2	0.87	29.00	21.89	0.0328	3.95	25.84
6	2	2	1	0.80	26.67	20.13	0.0225	2.71	22.84
7	2	3	4	1.25	41.67	31.45	0.0348	4.20	35.64
8	2	4	3	1.26	42.00	31.70	0.0076	0.92	32.61
9	3	1	3	1.33	44.33	33.46	0.0259	3.12	36.58
10	3	2	4	1.12	37.33	28.18	0.0060	0.72	28.90
11	3	3	1	1.03	34.33	25.91	0.0486	5.86	31.77
12	3	4	2	1.00	33.33	25.16	0.0247	2.98	28.13
13	4	1	4	1.06	35.33	26.67	0.0281	3.39	30.05
14	4	2	3	0.68	22.67	17.11	0.0900	10.85	27.96
15	4	3	2	0.97	32.33	24.40	0.0193	2.33	26.73
16	4	4	1	0.95	31.67	23.90	0.0860	10.37	34.27
K₁	60.01	43.78		42.88
K₂	29.23	38.76		22.29
K₃	31.35	36.81		37.56
K₄	29.75	30.99		30.89
R	0.78	12.79		20.59

产物	峰面积						平均值	RSD/%
产物	1	2	3	4	5	6	平均值	RSD/%
白藜芦醇	3638.987	3672.407	3682.220	3647.186	3584.484	3652.530	3646.302	0.94
白藜芦醇苷	113.618	112.469	114.125	110.829	112.542	112.634	112.703	0.92