Parameter estimation and cold model experiments of gastrointestinal simulation reactor

doi:10.11949/j.issn.0438-1157.20181488

Abstract

Abstract:

In vitro digestion and fermentation experiments should be closely combined to simulate the function of the intact gastrointestinal tract. However, the existing simulated reactors for gastrointestinal digestion and large intestinal fermentation have different structures and are not universal. Combining the separated reactors would increase the complexity and the cost of the experiments. Integrated the dominant features of both types of reactors, a gastrointestinal simulation reactor(GSR) using a programmable logic controller(PLC) was developed in the present work. The dynamic characteristics of overall gastrointestinal tract were simulated and compared to the data in vivo. It shows that the developed reactor can accurately simulate the dynamic characteristics of peristalsis, emptying and mixing in the stomach and small intestine, and provide a fermentation environment for intestinal microbes. The exhaust gas discharge and collection device was considerately designed, the exhaust gas was quickly discharged and the volume could be accurately measured. The gastrointestinal simulation reactor can help to explore the role of prebiotics in intestinal microbes in vitro.

Key words: gastrointestinal tract, simulation, reactor, PLC, design, in vitro

摘要：

体外消化和发酵实验紧密结合才能模拟完整消化道的功能，现有的模拟胃肠消化和大肠发酵的反应器结构各异且不通用，组合多个独立反应器则增加了实验的复杂程度和设备成本。集成了两类反应器的优势特征，开发了一种采用可编程逻辑控制器（PLC）的模拟胃肠道反应器（GSR），模拟了整体的胃肠道动态特征并与体内数据进行了比较。结果表明模拟胃肠道反应器既可准确模拟胃和小肠的蠕动、排空和混合等动态特征，又可提供肠道微生物的发酵环境。尾气排放和收集装置的设计实现了尾气快速排出和尾气体积的准确计量。模拟胃肠道反应器可为体外探索益生元对肠道微生物作用等研究提供帮助。

关键词: 胃肠道, 模拟, 反应器, PLC, 设计, 体外

CLC Number:

TQ 027.6

Wenlong ZHANG, Xiaobei ZHAN, Luo CHEN, Zhiyong ZHENG, Li ZHU, Zhitao LI, Minjie GAO. Parameter estimation and cold model experiments of gastrointestinal simulation reactor[J]. CIESC Journal, 2019, 70(5): 1879-1886.

张文龙, 詹晓北, 陈荦, 郑志永, 朱莉, 李志涛, 高敏杰. 模拟胃肠道反应器的参数建立与冷模实验研究[J]. 化工学报, 2019, 70(5): 1879-1886.

Figures/Tables 11

Fig.1 Schematic diagram of GSR

Fig.2 Exhaust gas discharge and collection device

Fig.3 Pressure profiles in reactors during simulating gastric peristalsis

Fig.4 Pressure profile in GSR during simulating intestinal peristaltic waves

Fig.5 Simulation of gastric and ileal emptying

Table 1 Parameters to describe curves for gastric and ileal emptying[7]

模型	快排空		慢排空
模型	t _1/2/min	β	t _1/2/min	β
胃	35	1.15	70	2
回肠	85	1.4	160	1.6

Table 2 Parameters for fitting emptying curve

模型	快排空			慢排空
模型	t _1/2/min	β	r ²	t _1/2/min	β	r ²
胃	35.09±0.18	1.18±0.01	0.9999	68.68±0.52	2.00±0.04	0.9995
回肠	84.10±0.03	1.43±0.00	0.9999	155.36±0.52	1.66±0.01	0.9997

Table 3 Average relative error between emptying curve and Elashoff’s model and average relative deviation of parallel experiments

模型		快排空		慢排空
模型		相对误差/%	相对偏差/%	相对误差/%	相对偏差/%
GSR	胃	0.66	0.20	2.12	1.15
GSR	回肠	1.29	0.21	3.04	0.53
TIM-1^[7]	胃	0.51	2.73	5.18	6.12
TIM-1^[7]	回肠	5.16	6.32	9.32	11.67

Fig.6 Performance of liquid mixing in GSR

Fig.7 Percentage of short-chain fatty acids remaining in GSR and TIM-2

Table 4 Performance of gas collection in GSR

注入空气体积/ml	气体停留时间/min	体积回收率/%
10.5	15.1±2.1	85.7±0.0
19.7	9.4±0.9	93.7±1.3
51.0	7.1±0.7	96.4±0.6

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