CIESC Journal ›› 2023, Vol. 74 ›› Issue (2): 617-629.DOI: 10.11949/0438-1157.20221223
• Reviews and monographs • Previous Articles Next Articles
Weiyi SU1,2(), Jiahui DING1,2, Chunli LI1,2, Honghai WANG1,2, Yanjun JIANG1,2()
Received:
2022-09-07
Revised:
2022-11-18
Online:
2023-03-21
Published:
2023-02-05
Contact:
Yanjun JIANG
苏伟怡1,2(), 丁佳慧1,2, 李春利1,2, 王洪海1,2, 姜艳军1,2()
通讯作者:
姜艳军
作者简介:
苏伟怡(1985—),女,博士,副教授,suweiyi@hebut.edu.cn
基金资助:
CLC Number:
Weiyi SU, Jiahui DING, Chunli LI, Honghai WANG, Yanjun JIANG. Research progress of enzymatic reactive crystallization[J]. CIESC Journal, 2023, 74(2): 617-629.
苏伟怡, 丁佳慧, 李春利, 王洪海, 姜艳军. 酶促反应结晶研究进展[J]. 化工学报, 2023, 74(2): 617-629.
酶 | 目标产物 | 结晶方式 | 调控参数 | 产率 | 文献 |
---|---|---|---|---|---|
转氨酶 | 1-苯基乙胺 | 加成盐剂 | 75% | [ | |
加成盐剂 | pH、温度 | 91.5% | [ | ||
(S)-1-(3-甲氧基苯基)乙胺 | 加成盐剂 | 晶种 | [ | ||
加成盐剂 | 晶种 | [ | |||
3-(2-萘)- l -丙氨酸 | 自发结晶 | 温度 | 93% | [ | |
L-邻苯丙氨酸 | 自发结晶 | pH | [ | ||
青霉素G酰化酶 | 氨苄西林 | 自发结晶 | 93% | [ | |
自发结晶 | 97% | [ | |||
自发结晶 | pH | 96% | [ | ||
加入晶种 | 晶种 | [ | |||
自发结晶 | 晶种、pH | [ | |||
自发结晶 | pH | 98% | [ | ||
阿莫西林 | 自发结晶 | pH | 98% | [ | |
头孢氨苄 | 加络合剂 | 温度、pH | [ | ||
头孢克洛 | 加络合剂 | 温度、pH | 80% | [ | |
头孢拉定 | 加络合剂 | [ | |||
葡萄糖氧化酶 | 葡萄糖酸钙 | 自发结晶 | 温度、pH | [ | |
氨基酸消旋酶 | 苏氨酸 | 自发结晶 | [ | ||
芳香酸脱羧酶 | 2,6-二羟基苯甲酸 | 加成盐剂 | 97% | [ | |
嗜热菌蛋白酶 | Z-阿斯巴甜 | 自发结晶 | pH | 88% | [ |
延胡索酸酶 | L-苹果酸钙 | 自发结晶 | 温度 | [ |
Table 1 Summary of the realization and regulation of enzymatic reaction crystallization
酶 | 目标产物 | 结晶方式 | 调控参数 | 产率 | 文献 |
---|---|---|---|---|---|
转氨酶 | 1-苯基乙胺 | 加成盐剂 | 75% | [ | |
加成盐剂 | pH、温度 | 91.5% | [ | ||
(S)-1-(3-甲氧基苯基)乙胺 | 加成盐剂 | 晶种 | [ | ||
加成盐剂 | 晶种 | [ | |||
3-(2-萘)- l -丙氨酸 | 自发结晶 | 温度 | 93% | [ | |
L-邻苯丙氨酸 | 自发结晶 | pH | [ | ||
青霉素G酰化酶 | 氨苄西林 | 自发结晶 | 93% | [ | |
自发结晶 | 97% | [ | |||
自发结晶 | pH | 96% | [ | ||
加入晶种 | 晶种 | [ | |||
自发结晶 | 晶种、pH | [ | |||
自发结晶 | pH | 98% | [ | ||
阿莫西林 | 自发结晶 | pH | 98% | [ | |
头孢氨苄 | 加络合剂 | 温度、pH | [ | ||
头孢克洛 | 加络合剂 | 温度、pH | 80% | [ | |
头孢拉定 | 加络合剂 | [ | |||
葡萄糖氧化酶 | 葡萄糖酸钙 | 自发结晶 | 温度、pH | [ | |
氨基酸消旋酶 | 苏氨酸 | 自发结晶 | [ | ||
芳香酸脱羧酶 | 2,6-二羟基苯甲酸 | 加成盐剂 | 97% | [ | |
嗜热菌蛋白酶 | Z-阿斯巴甜 | 自发结晶 | pH | 88% | [ |
延胡索酸酶 | L-苹果酸钙 | 自发结晶 | 温度 | [ |
Fig.2 In situ product crystallization (ISPC) was combined with the reaction catalyzed by aminotransferase to form insoluble 1-phenylethylamine salt[18]
Fig.3 Schematic diagram of a transaminases-catalyzed continuous reaction process [containing the dissolution of donor salts (left) and crystallization of the product salts (right)][20]
Fig.5 The schematic pathway for the creation of supersaturated solution(solid line is the thermodynamic solubility of 6-APA, and point 3 corresponds to 6-APA concentration in a supersaturated solution)[48]
Fig.7 Flow chart of cefaclor enzymatic reactive crystallization (complexation crystallization) (left is the composite reactor, and right is the enzyme reactor)[32]
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