CIESC Journal ›› 2015, Vol. 66 ›› Issue (8): 2846-2853.doi: 10.11949/j.issn.0438-1157.20150948

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Preparation and structure control of macroporous polymer microspheres

ZHOU Weiqing, LI Juan, NA Xiangming, MA Guanghui   

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2015-06-19 Revised:2015-06-23 Online:2015-08-05 Published:2015-06-30
  • Supported by:

    supported by the National Natural Science Foundation of China (21336010) and the National High Technology Research and Development Program of China (2012AA02A406, 2012AA021505).


Formation and structure control of macroporous microsphere is a complex process. Special techniques were developed for the preparation of gigaporous microspheres with large pore size over 100 nm. To meet the needs of gigaporous microsphere in biochemical engineering, the reverse micelles swelling method and the multiple emulsion method were developed which achieved the control of pore diameter above 100 nm. The effects of microsphere structures on their applications were also studied. When used in separation and purification of biomacromolecule, the gigaporous microspheres showed much higher capacity, activity recovery and purification fold. Immobilized enzymes in gigaporous microsphere also have some significant advantages in thermal stability, storage stability and reusability. The effective control of the microsphere structure is important for success application in different fields.

Key words: macroporous microsphere, structure control, drug controlled release, separation and purification

CLC Number: 

  • TQ316.3

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