Resin adsorption - ultrafiltration synergistic separation of alkaline extracted hemicellulose from bagasse

doi:10.11949/0438-1157.20201172

Abstract

Abstract:

Aiming at the problem of high lignin content in the hemicellulose extracted by alkaline method and difficult purification, the hydrophobic resin adsorption-ultrafiltration is used for cooperative treatment. In this study, the effect of different methods on the purification and separation of hemicellulose was explored through the method of macroporous resin adsorption and ultrafiltration technology. Ion chromatography (IC), ultraviolet spectrophotometer (UV), gel permeation chromatography (GPC) and thermogravimetric analysis (TGA) were used to analyze chemical composition, molecular weight, thermal stability and other characteristics of the separated and purified hemicellulose. The results show that the resin adsorption-membrane ultrafiltration treatment can effectively remove lignin and obtain hemicellulose with higher molecular weight. No significant changes are found in the molecular backbone structure, and the thermal stability is slightly improved. The side chain unit of hemicellulose after resin adsorption has obvious changes. The hemicellulose with smaller molecular weight has more side chain lignin unit structure, and is mostly connected to carbohydrates by phenyl glycosidic bonds (PhGlc). Through the cooperative treatment of resin adsorption and ultrafiltration, hemicellulose with different side chain units and lignin content can be obtained, which provides a basis for expanding the utilization value of hemicellulose.

Key words: hemicellulose, lignin, membrane separation, XAD resin, bagasse, separation and purification

摘要：

针对碱法提取半纤维素中木质素含量高、纯化难的问题，通过疏水树脂吸附-超滤协同处理，探讨纯化分离方式的影响。利用离子色谱（IC）、紫外分光光度计（UV）、凝胶渗透色谱（GPC）和热重量分析（TGA），对半纤维素的化学组分、分子量、热稳定性等特性进行了分析。结果表明，树脂吸附-膜超滤处理能有效脱除木质素，获得较高分子量的半纤维素，分子主链结构没有发现明显变化，热稳定性略有提高。经树脂吸附后的半纤维素侧链单元有较为明显的变化，分子量较小的半纤维素带有更多侧链木质素单元，通过苯基糖苷键（PhGlc）与碳水化合物相连，可通过树脂吸附-超滤协同处理分离出来，从而达到分离不同侧链结构的半纤维素。

关键词: 半纤维素, 木质素, 膜分离, XAD树脂, 甘蔗渣, 分离纯化

CLC Number:

TQ 353

DU Juan, GONG Zhiqiang, HUANG Caoxing, LIANG Chen, YAO Shuangquan, LIU Yang. Resin adsorption - ultrafiltration synergistic separation of alkaline extracted hemicellulose from bagasse[J]. CIESC Journal, 2021, 72(4): 2139-2147.

杜娟, 龚志强, 黄曹兴, 梁辰, 姚双全, 刘杨. 树脂吸附-超滤协同分离甘蔗渣碱法半纤维素[J]. 化工学报, 2021, 72(4): 2139-2147.

Figures/Tables 11

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糖组分	Hu₂/%	Hu₃/%	Hu₄/%	Hu₅/%	Hu₆/%
木糖	62.81	56.14	53.16	50.25	39.56
阿拉伯糖	14.36	14.78	14.96	11.98	11.58
葡萄糖	3.74	2.76	3.03	3.14	2.44
半乳糖	3.81	4.17	3.38	3.32	3.39
甘露糖	2.01	1.43	1.58	2.14	1.29
木质素	1.51	2.39	3.77	5.82	17.06
糠醛	4.77	4.32	4.12	3.99	2.94
甲酸	10.05	12.58	10.05	9.27	8.68
乙酸	3.10	3.44	3.91	2.75	3.14

糖组分	Hu₂/%	Hu₃/%	Hu₄/%	Hu₅/%	Hu₆/%
木糖	62.81	56.14	53.16	50.25	39.56
阿拉伯糖	14.36	14.78	14.96	11.98	11.58
葡萄糖	3.74	2.76	3.03	3.14	2.44
半乳糖	3.81	4.17	3.38	3.32	3.39
甘露糖	2.01	1.43	1.58	2.14	1.29
木质素	1.51	2.39	3.77	5.82	17.06
糠醛	4.77	4.32	4.12	3.99	2.94
甲酸	10.05	12.58	10.05	9.27	8.68
乙酸	3.10	3.44	3.91	2.75	3.14

糖组分	Hau₂/%	Hau₃/%	Hau₄/%	Hau₅/%	Hau₆/%
木糖	56.97	56.93	51.61	52.96	38.70
阿拉伯糖	14.00	11.11	11.07	9.06	11.58
葡萄糖	3.49	2.46	3.63	2.86	2.54
半乳糖	3.78	1.70	3.69	1.94	3.39
甘露糖	1.92	1.86	2.66	2.27	1.69
木质素	1.39	1.86	2.18	2.53	14.86
糠醛	4.47	4.31	3.87	3.93	2.87
甲酸	11.49	15.47	11.37	14.51	9.36
乙酸	3.65	4.51	3.83	2.96	2.48

糖组分	Hau₂/%	Hau₃/%	Hau₄/%	Hau₅/%	Hau₆/%
木糖	56.97	56.93	51.61	52.96	38.70
阿拉伯糖	14.00	11.11	11.07	9.06	11.58
葡萄糖	3.49	2.46	3.63	2.86	2.54
半乳糖	3.78	1.70	3.69	1.94	3.39
甘露糖	1.92	1.86	2.66	2.27	1.69
木质素	1.39	1.86	2.18	2.53	14.86
糠醛	4.47	4.31	3.87	3.93	2.87
甲酸	11.49	15.47	11.37	14.51	9.36
乙酸	3.65	4.51	3.83	2.96	2.48

Sample	M_w	M_n	M_w/M_n
Hu₂	4.57×10⁴	4.11×10⁴	1.11
Hu₆	6.78×10⁴	3.92×10⁴	1.73
Hau₂	8.54×10⁴	5.16×10⁴	1.65
Hau₆	6.47×10⁴	4.48×10⁴	1.44