]*>","")" /> 聚合脂质体亲和沉淀纯化胰蛋白酶

CIESC Journal

• 化工学报 • 上一篇    下一篇

聚合脂质体亲和沉淀纯化胰蛋白酶

董晓燕,孙彦,于凯,金仙华   

  1. 天津大学化学工程系!天津300072,天津大学化学工程系!天津300072,天津大学化学工程系!天津300072,天津大学化学工程系!天津300072
  • 出版日期:1996-04-25 发布日期:1996-04-25

TRYPSIN PURIFICATION BY AFFINITY PRECIPITATION WITH POLYMERIZED LIPOSOME AS LIGAND CARRIER

Dong Xiaoyan;Sun Yan;Yu Kai;Jin Xianhua(Department of Chemical Engineering,Tianjin University,Tianjin300072)   

  • Online:1996-04-25 Published:1996-04-25

Abstract: A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg~(-1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.

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