关键词: 丁醇, Cl.beijerinckii, 原生质体, ⁶⁰Co-γ射线, 诱变

Abstract:

A mutant Clbeijerinckii strain with higher butanol production ability was obtained by protoplast mutation with ⁶⁰Co-γ irraditionThe optimal conditions for protoplast preparation were: the cell cultivated for 15 h was dealt with 1 g.L^-1 glycin and 0.3 U.L^-1 penicillin for 2 h and then digested with 1×10⁴ U.L^-1 lysozyme at 37℃ for 8 h.By plate count method, the formation and the regeneration rate of protoplasts was calculated as 99.82% and 10.12% respectively.The protoplasts were then irradiated by ⁶⁰Co with a dose rate of 5Gy.min^-1.The lethality of the protoplasts was 99.3% when the radiation dose was 500 Gy.Under such condition, a mutant strain R₃ with higher butanol production ability and higher inheritance stability was selectedCompared with the original strain, the total solvent concentration and the butanol concentration of the mutant strain R₃ was increased by 81.46% and 85.41% respectively in the P₂ semi-synthesis medium at 37℃.

Key words: 丁醇, Cl.beijerinckii, 原生质体, ⁶⁰Co-γ射线, 诱变