粘质沙雷氏菌产灵菌红素的碳源分析、菌种诱变及动力学

doi:10.11949/j.issn.0438-1157.20160505

化工学报 ›› 2017, Vol. 68 ›› Issue (1): 244-255.DOI: 10.11949/j.issn.0438-1157.20160505

粘质沙雷氏菌产灵菌红素的碳源分析、菌种诱变及动力学

荣广健¹, 张佑红², 陈艳², 谌颉², 黄萌², 周锋²

1 武汉工程大学化学与环境工程学院, 湖北武汉 430073;
2 武汉工程大学化工与制药学院, 绿色化工过程教育部重点实验室, 湖北武汉 430073

收稿日期:2016-04-19 修回日期:2016-10-10 出版日期:2017-01-05 发布日期:2017-01-05
通讯作者: 张佑红
基金资助:
湖北省科技厅省重点自然科学基金（2013CFA101）。

Carbon source analysis, mutagenesis and kinetics of Serratia marcescens producing prodigiosin

RONG Guangjian¹, ZHANG Youhong², CHEN Yan², CHEN Jie², HUANG Meng², ZHOU Feng²

1 School of Chemistry Environmental Engineering, Wuhan Institute of Technology, Wuhan 430073, Hubei, China;
2 School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan 430073, Hubei, China

Received:2016-04-19 Revised:2016-10-10 Online:2017-01-05 Published:2017-01-05
Contact: 10.11949/j.issn.0438-1157.20160505
Supported by:
supported by Hubei Key Natural Science Foundation, China (2013CFA101).

摘要/Abstract

摘要：

使用流式细胞仪研究了不同碳源对粘质沙雷氏菌ZSG新陈代谢的影响，发现不同碳源导致ZSG的DNA含量、细胞内部颗粒密度、表面粗糙度和细胞大小在发酵过程中呈现有差异的变化。对ZSG进行复合诱变筛得一株稳定突变菌株ZSG7，在250 ml摇瓶和5 L发酵罐中进行发酵，其灵菌红素（PG）产量比出发菌株分别提高了62.5%和269%。对ZSG7进行发酵培养基优化后PG产量比优化前提高了100%。对ZSG7发酵进行溶氧分段控制模式调控后PG产量比DO调控前提高了30.9%。对ZSG7发酵进行恒定pH调控后比pH调控前提高了35.9%。对ZSG7发酵进行补料组分优化后比补料前提高了47.6%。基于Logistic方程和Luedeking-Piret方程建立了恒定pH7分批发酵和补料分批发酵的菌体生长模型和PG合成模型。拟合模型参数后，模型可以合理地描述恒定pH7分批发酵和补料分批发酵的过程。

关键词: 流式细胞仪, 碳源, 灵菌红素, 动力学模型, 诱变, 优化, 发酵

Abstract:

The DNA contents, the internal grain densities, the surface roughness and the cell sizes of Serratia marcescens ZSG cells were detected to have different changes by the flow cytometry when ZSG cells were cultivated in the medium with the different carbon sources.Through mutagenizing compoundly ZSG cells, a stable mutant strain ZSG7 was gained, of which the prodigiosin(PG) production in the 250 ml conical flask and in 5 L fermentor were increased by 62.5% and 269% compared with the the original strain yields, respectively.PG production of fermentation with the optimal culture medium in the 250 ml conical flask was increased by 100% compared with the unoptimized.After dissolved oxygen(DO) was optimized by step controlling, the PG production was increased by 30.9% compared with the DO-unoptimized.After constant pH was optimized, the PG production was increased by 35.9% compared with the pH-unoptimized.After the fed-batch components were optimized, the PG production was increased by 47.6% compared with the batch fermentation with constant pH7.The kinetic models about bacteria growth and PG formation of the batch fermentation with constant pH7 and the fed-batch fermentation were built based on the Logistic equation and Luedeking-Piret equation.With the fitting model parameters, the models could provide reasonable description for the processes of the batch fermentation with constant pH7 and the fed-batch fermentation.

Key words: flow cytometry, carbon source, prodigiosin, kinetic modeling, mutagenesis, optimization, fermentation

中图分类号:

Q815
TQ920.6

荣广健, 张佑红, 陈艳, 谌颉, 黄萌, 周锋. 粘质沙雷氏菌产灵菌红素的碳源分析、菌种诱变及动力学[J]. 化工学报, 2017, 68(1): 244-255.

RONG Guangjian, ZHANG Youhong, CHEN Yan, CHEN Jie, HUANG Meng, ZHOU Feng. Carbon source analysis, mutagenesis and kinetics of Serratia marcescens producing prodigiosin[J]. CIESC Journal, 2017, 68(1): 244-255.

参考文献 21

[1]	MONTANER B, PREZ-TOMS R. The prodigiosins:a new family of anticancer drugs[J]. Current Cancer Drug Targets, 2003, 3(1):57-65.
[2]	ARAUJO A J, MARINHO F J, SOUSA T S, et al. Evidences for the involvement of HER on prodigiosin anticancer effects[J]. Planta Medica, 2012, 78(11):78-92.
[3]	KAVITHA R, AISWARIYA S, RATNAVALI M G. Anticancer activity of red pigment from Serratia marcescens in human cervix carcinoma[J]. International Journal of PharmTech Research, 2010, 2(1):784.
[4]	CHAWRAI S R, WILLIAMSON N R, MAHENDIRAN T, et al. Characterisation of Pig C and Hap C, the prodigiosin synthetases from Serratia sp. and Hahella chejuensis with potential for biocatalytic production of anticancer agents[J]. Chemical Science, 2012, 3:447-454.
[5]	ALESSIO D R, BARGIOTTI A, CARLINI O, et al. Synthesis and immunosuppressive activity of novel prodigiosin derivatives[J]. Journal of Medicinal Chemistry, 2000, 43:2557-2565.
[6]	王玉洁, 孙诗清, 朱长俊, 等. 天然红色素灵菌红素的抗菌性能及应用[J]. 天然产物研究与开发, 2012, 24:1626-1629, 1654. WANG Y J, SUN S Q, ZHU C J, et al. Antibacterial property and application of natural red pigment prodigiosin[J]. Natural Product Research and Development, 2012, 24:1626-1629, 1654.
[7]	NAKASHIMA T, KATO Y, YAMAGUCHI K. Evaluation of the anti-trichophyton activity of a prodigiosin analogue produced by γ-proteobacterium, using stratum corneum epidermis of the Yucatan micropig[J]. Journal of Infection and Chemotherapy, 2005, 11(3):123-128.
[8]	ISAKA M, JATURAPAT A, KRAMYU J, et al. Potent in vitro antimalarial activity of metacycloprodigiosin isolated from Streptomyces spectabilis BCC 4785[J]. Antimicrobial Agents and Chemotherapy, 2002, 46(4):1112-1113.
[9]	GERBER N N. Prodigiosin-like pigments[J]. Critical Reviews in Microbiology, 1975, 3:469-485.
[10]	JEONG H, YIM J H, LEE C, et al. Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent[J]. Nucleic Acids Research, 2005, 33(22):7066-7073.
[11]	ANA K, TATJANA I T, MILOS P, et al. Crude bacterial extracts of two new Streptomyces sp. isolates as bio-colorants for textile dyeing[J]. World Journal of Microbiology & Biotechnology, 2014, 30(8):2231-2240.
[12]	刘同军, 杨海龙, 唐华. 灵菌红素的研究进展[J]. 食品与药品, 2007, 9(8):47-51. LIU T J, YANG H L, TANG H. Progress on prodigiosin[J]. Food and Drug, 2007, 9(8):47-51.
[13]	EL-BONDKLY A M, EL-GENDY M M, BASSYOUNI R H. Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species[J]. Antonie Van Leeuwenhoek, 2012, 102(4):719-734.
[14]	GUTIÉRREZ-ROMÁN M I, HOLGUIN-MELÉNDEZ F, BELLO-MENDORA R, et al. Production of prodigiosin and chitinases by tropical Serratia marcescens strains with potential to control plant pathogens[J]. World Journal of Microbiology and Biotechnology, 2012, 28(1):145-153.
[15]	SAKATA T, YOSHIKAWA T, NISHITARUMIZU S. Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp. C55a-2[J]. Fisheries Science, 2011, 77(3):397-402.
[16]	CUI Y H, TONG Q Y, WU S J. Kinetic model of pullulan batch fermentation[J]. Journal of Food Science and Biotechnology, 2009, 28(3):361-365.
[17]	张峻峰, 刘道亮, 赵占民, 等. 流式细胞术在微生物快速检测领域的研究进展[J]. 食品工程, 2010, (3):19-22. ZHANG J F, LIU D L, ZHAO Z M, et al. Research development on flow cytometry in the rapid detection of microorganisms[J]. Food Engineering, 2010, (3):19-22.
[18]	朱雄伟, 翟莉莉, 张楠, 等. 产灵菌红素菌株的分离鉴定[J]. 湖北大学学报(自然科学版), 2012, 34(4):384-386,393. ZHU X W, ZHAI L L, ZHANG N, et al. Separation and identification of prodigiosin-producing strain[J]. Journal of Hubei University (Natural Science), 2012, 34(4):384-386,393.
[19]	陈艳, 谌颉, 张佑红, 等. 灵菌红素分批发酵过程的溶氧和补料调控[J]. 化学与生物工程, 2015, (8):56-59. CHEN Y, CHEN J, ZHANG Y H, et al. Regulation of DO and feeding in process of prodigiosin batch fermentation[J]. Chemistry & Bioengineering, 2015, (8):56-59.
[20]	戚以政, 汪叔雄. 生化反应动力学与反应器[M]. 北京:化学工业出版社, 2007:40. QI Y Z, WANG S X. The Biochemical Reaction Kinetics and Reactor[M]. Beijing:Chemical Industry Press, 2007:40.
[21]	LUEDEKING R, PIRET E L. A kinetic study of the lactic acid fermentation:batch process at controlled pH[J]. J. Biochem. Microbiol. Technol. Eng., 1959, 1(4):393.

粘质沙雷氏菌产灵菌红素的碳源分析、菌种诱变及动力学

Carbon source analysis, mutagenesis and kinetics of Serratia marcescens producing prodigiosin

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