化工进展

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木聚糖酶高产菌筛选研究进展

何敏超,张 宇,许敬亮,袁振宏   

  1. 中国科学院广州能源研究所,中国科学院可再生能源与天然气水合物重点实验室,广东 广州 510640
  • 出版日期:2013-01-05 发布日期:2013-01-25

Research progress of screening of high xylanase-producing strain

HE Minchao, ZHANG Yu, XU Jingliang, YUAN Zhenhong   

  1. Key Laboratory of Renewable Energy and Gas Hydrate, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China
  • Online:2013-01-05 Published:2013-01-25

摘要: 从自然界筛选具有工业化应用潜力的高酶活产酶菌株,对于满足科学研究和实际生产需要都具有重要的意义。本文按照不同酶活梯度与采样点的关系,总结含有高酶活菌株样品的一些特征,如:富含腐烂木质纤维素的土样,该采样点的地势较平坦等。此外,对筛选到的高活力菌株的微生物种类及发酵优化中碳源的重要性进行了概括,提出最佳碳源的正确选择对最终的发酵优化工艺具有极大影响作用。对木聚糖酶高产菌株的筛选及发酵优化提供了有益的参考。

关键词: 木聚糖酶, 菌株筛选, 碳源

Abstract: To satisfy the requirement of research and production, it is important and necessary to obtain high xylanase-producing strain, which has great application potential of commercialization. Drawn from the relationship between enzyme activity and the site of sampling, we could draw some valuable conclusions about strain screening of high xylanase activity. For example, the samples should be taken from ligonocellulose rich and flat locations. In addition, we also reviewed the species of high xylanase activity producing microbe and the importance of carbon source optimization. This paper could provide some significant inferences about the screening of high yielding strain and fermentation optimization.

Key words: xylanase, strain screening, carbon source