基于侧流富集/主流强化的CANON工艺处理常温低氨氮废水的稳态控制

doi:10.11949/j.issn.0438-1157.20170663

化工学报 ›› 2017, Vol. 68 ›› Issue (12): 4723-4730.DOI: 10.11949/j.issn.0438-1157.20170663

基于侧流富集/主流强化的CANON工艺处理常温低氨氮废水的稳态控制

曹丽娟, 陈杰, 姜广萌, 常青龙, 王亚宜

同济大学环境科学与工程学院, 污染控制与资源化研究国家重点实验室, 上海 200092

收稿日期:2017-05-22 修回日期:2017-07-15 出版日期:2017-12-05 发布日期:2017-12-05
通讯作者: 王亚宜
基金资助:
国家自然科学基金（优秀青年基金）项目（51522809）。

Controlling strategy for a CANON system treating wastewater with low ammonium concentration at room temperature via bio-augmentation batch enhance method

CAO Lijuan, CHEN Jie, JIANG Guangmeng, CHANG Qinglong, WANG Yayi

State Key Laboratory of Pollutant Controlling and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China

Received:2017-05-22 Revised:2017-07-15 Online:2017-12-05 Published:2017-12-05
Supported by:
supported by the National Natural Science Foundation of China (Outstanding Young Foundation)(51522809).

摘要/Abstract

摘要：

采用侧流富集/主流强化方式，研究了全程自养脱氮工艺（CANON）用于常温（25℃）、低氨氮（约60 mg NH₄⁺-N·L^-1）主流线生物脱氮的可行性。结果表明，通过7 d更换主流反应器40%污泥混合液方式可实现维持主流反应器总氮去除负荷（TNRR）在80 g N·m^-3·d^-1左右，总氮去除率（TNRE）在70%左右，主流反应器厌氧氨氧化比活性持续升高。16S rDNA高通量测序结果表明，主流和侧流CANON系统中起亚硝化作用的氨氧化细菌（AOB）主要是Nitrosomonas属，进行Anammox反应的厌氧氨氧化菌（AnAOB）主要是Candidatus Jettenia属，60 d的运行过程中主流反应器Nitrospira属的亚硝酸盐氧化菌（NOB）丰度始终小于1%。可见在本实验条件下，采用7 d为频率主流和侧流换泥方式，能够保证主流反应器中Anammox活性，确保主流CANON反应器的脱氮性能。

关键词: CANON, 侧流富集/主流强化, 常温低氨氮, 高通量测序

Abstract:

The feasibility of the completely autotrophic nitrogen removal over nitrite (CANON) process for removal of nitrogen from mainstream wastewater (~60 mg NH₄⁺-N·L^-1) at room temperature (25℃) was investigated using bio-augmentation batch enhanced (BABE) controlling strategy. The results indicated that through changing 40% sludge of the mainstream reactor from sidestream reactor every 7 d, the total nitrogen removal rate (TNRR) and the total nitrogen removal efficiency (TNRE) stabilized at about 80 g N·m^-3·d^-1 and 70%.The specific anammox activity in mainstream reactor increased continuously. 16S rDNA gene high-throughput sequencing results showed that the functional microorganisms involved in both mainstream and sidestream reactor were Candidatus Jettenia like anammox bacteria and Nitrosomonas like ammonia-oxidizing bacteria (AOB), and the abundance of Nitrospira like nitrite-oxidizing bacteria (NOB) always maintained below 1% during 60 days' operation. It can be concluded that the operational mode of changing sludges between mainstream and sidestream every 7 d guarantees anammox activity and good nitrogen removal performance of mainstream CANON reactor under the experimental conditions herein.

Key words: CANON, bio-augmentation batch enhanced, room temperature and low ammonium concentration, high-throughput sequencing

中图分类号:

X703.1

曹丽娟, 陈杰, 姜广萌, 常青龙, 王亚宜. 基于侧流富集/主流强化的CANON工艺处理常温低氨氮废水的稳态控制[J]. 化工学报, 2017, 68(12): 4723-4730.

CAO Lijuan, CHEN Jie, JIANG Guangmeng, CHANG Qinglong, WANG Yayi. Controlling strategy for a CANON system treating wastewater with low ammonium concentration at room temperature via bio-augmentation batch enhance method[J]. CIESC Journal, 2017, 68(12): 4723-4730.

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基于侧流富集/主流强化的CANON工艺处理常温低氨氮废水的稳态控制

Controlling strategy for a CANON system treating wastewater with low ammonium concentration at room temperature via bio-augmentation batch enhance method

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