高通量测序技术辅助筛选脱硫菌

doi:10.3969/j.issn.0438-1157.2014.05.035

化工学报

高通量测序技术辅助筛选脱硫菌

徐瑛^1,2, 孙永明¹, 郑涛³, 袁振宏¹, 李颖¹, 孔晓英¹, 李志兵¹

1 中国科学院广州能源研究所, 广东广州 510640;
2 中国科学院大学, 北京 100049;
3 南京工业大学生物工程与制药学院, 江苏南京 210009

收稿日期:2013-12-31 修回日期:2014-03-04 出版日期:2014-05-05 发布日期:2014-05-05
通讯作者: 孙永明
基金资助:
中国科学院重点部署项目（KGZD-EW-304-1）。

Screening strains of desulfurization bacteria by high-throughput sequencing

XU Ying^1,2, SUN Yongming¹, ZHENG Tao³, YUAN Zhenhong¹, LI Ying¹, KONG Xiaoying¹, LI Zhibing¹

1 Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China;
2 Graduate University of Chinese Academy Sciences, Beijing 100049, China;
3 School of Biotechnology Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China

Received:2013-12-31 Revised:2014-03-04 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the Key Program of Chinese Academy of Sciences(KGZD-EW-304-1).

摘要/Abstract

摘要： 将脱硫菌的筛选与新一代高通量测序技术相结合，探索脱硫菌筛选的新方法，为生物甲烷的高值化利用提供技术支持。实验将富含硫细菌的环境土样及经过培养基富集培养后的液样分别进行16S rDNA V6区的高通量测序，并分析了物种组成和丰度、Alpha多样性和菌群结构。结果表明，高通量测序的平均数据利用率达99.177%，测序量能够充分反映样品在该区域细菌的群落组成和结构。取自环境的土壤样品物种组成丰富，含有Pseudomonadales（假单胞菌目）、Rhizobiales（根瘤菌目）、Desulfuromonadales（除硫单孢菌目）、Desulfobacterales（脱硫杆菌目）、Acidithiobacillales（酸硫杆状菌目）等脱硫菌目。经过培养基富集后，菌群多样性显著降低，主要组成菌为Pseudomonadales（假单胞菌目）和Rhizobiales（根瘤菌目）。通过高通量测序技术，能够在筛选脱硫菌之前就了解实验样品的菌群结构和组成，为有针对性地设计筛选脱硫菌实验提供了一种新的方法。

关键词: 生物甲烷, 生物分离, 生物分子工程, 高通量测序, 脱硫菌, 纯化

Abstract: In order to provide technical support for high-value utilization of bio-methane, the traditional screening method was combined with high-throughput sequencing to develop a new method for screening strains of desulfurization bacteria. Soil samples were taken from the environment rich in desulfurization bacteria; then the same samples were treated with enriched medium. High-throughput sequencing of samples were performed in the V6 region of 16S rDNA to analyze species content and abundance, alpha diversity and species structures of a single group. The average data utilization ratio of high-throughput sequencing was 99.177%, adequately reflecting the samples' species content and structures of the V6 region. The species composition of soil samples was rich, including some desulfurization bacteria, such as Pseudomonadales, Rhizobiales, Desulfuromonadales, Desulfobacterales and Acidithiobacillales. Species screened from enriched medium mainly contained Pseudomonadales and Rhizobiales, and lower species diversity was observed. The high-throughput sequencing technology provided a more selective method in bacteria screening and enhanced understanding of the samples' species content and structure before screening desulfurization bacteria.

Key words: biomethane, bio-separation, biomolecular engineering, high-throughput sequencing, desulfurization bacteria, purification

中图分类号:

徐瑛, 孙永明, 郑涛, 袁振宏, 李颖, 孔晓英, 李志兵. 高通量测序技术辅助筛选脱硫菌[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.035.

XU Ying, SUN Yongming, ZHENG Tao, YUAN Zhenhong, LI Ying, KONG Xiaoying, LI Zhibing. Screening strains of desulfurization bacteria by high-throughput sequencing[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.035.

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高通量测序技术辅助筛选脱硫菌

Screening strains of desulfurization bacteria by high-throughput sequencing

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