关键词: 蒲公英, 总黄酮, 双水相, 分配, 提取

Abstract: Total flavonoids in Taraxacum mongolicum are a highly effective medicine，and aqueous two-phase extraction process is an effective technique for extracting biomaterials from plant. Effect of molecular weight and mass fraction of PEG，mass fraction of (NH4)2SO4，temperature and pH of aqueous two-phase system were investigated during extraction of total flavonoids from Taraxacum mongolicum. The optimal extraction conditions were obtained through orthogonal experiments. The optimal condition thus obtained is as follows：the average molecular weight of PEG at 1000，the mass fraction of PEG1000 at 23%，the mass fraction of (NH4)2SO4 at 18%，temperature at 25℃，pH at 5.34. The NaCl addition has little effect on flavonoids partition. The extraction yield of total flavonoids from Taraxacum mongolicum can reach up to 5.47% under this optimal condition. Compared with other extraction methods，the aqueous two-phase extraction process is a more environment-friendly and highly efficient technique.

Key words: Taraxacum mongolicum, total flavonoids, aqueous two-phase system, partition, extraction