CIESC Journal ›› 2022, Vol. 73 ›› Issue (11): 5128-5137.DOI: 10.11949/0438-1157.20221125

• Energy and environmental engineering • Previous Articles     Next Articles

Analysis of bacterial function in combined PN/A granular sludge and solid phase denitrification processes

Li ZHANG1(), Jianhua WU1, Shuhui CUI1, Feng YAN1, Hao SUN3, Feiyue QIAN1,2()   

  1. 1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, China
    2.National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, Jiangsu, China
    3.Suzhou Hongyu Environmental Poltron Technologies Incorporated, Suzhou 215009, Jiangsu, China
  • Received:2022-08-08 Revised:2022-10-12 Online:2022-12-06 Published:2022-11-05
  • Contact: Feiyue QIAN

PN/A颗粒污泥-固相反硝化组合工艺的菌群功能解析

张立1(), 吴建华1, 崔舒惠1, 严锋1, 孙浩3, 钱飞跃1,2()   

  1. 1.苏州科技大学环境科学与工程学院,江苏 苏州 215009
    2.城市生活污水资源化利用技术国家地方联合工程实验室,江苏 苏州 215009
    3.苏州市宏宇环境科技股份有限公司,江苏 苏州 215009
  • 通讯作者: 钱飞跃
  • 作者简介:张立(1998—),男,硕士研究生,leez048@post.usts.edu.cn
  • 基金资助:
    江苏省自然科学基金项目(BK20211339);江苏高校自然科学基金面上项目(21KJB610016);苏州市社会发展科技创新项目(SS202114);苏州市建设科技项目(SZJ202129503)

Abstract:

For the control of residual nitrate in the effluent of partial nitritation and anaerobic ammonium oxidation (PN/A) system, a novel system was developed for the advanced nitrogen removal by coupling solid-phase denitrification (SPD) and PN/A granular sludge. On the basis of increasing the autotrophic denitrification efficiency of granular sludge step by step, the SPD of three fillers, quartz sand, polybutylene succinate (PBS) and hydroxybutyrate valeric acid copolyester (PHBV), was systematically investigated. The results indicated that the denitrification performance of PHBV was the best, the optimal total nitrogen (TN) removal efficiency exceed 93% in the PN/A-PHBV system, which was significantly higher than sand (83.6%) and PBS (85.8%) in high hydraulic pressure condition. According to the results of pyrosequencing analysis and the FAPROTAX function predicted, the dissolution level of solid carbon source is an important factor affecting the diversity of biofilm flora. Among them, the hydrolytic bacteria Clostridium-sensu-stricto-7, which was dominant on the surface of PHBV, formed a stable cooperative relationship with denitrifying bacteria such as Comamonadaceae, in addition to effectively eluting autotrophic denitrification functional bacteria, the functional potential of the flora to participate in multi-element cycling was significantly improved. The results of this study have significance in guiding for systematic optimization of the PN/A-SPD combination process and promoting its engineering application.

Key words: autotrophic biological nitrogen removal, granular sludge, solid carbon source, denitrification, bacterial community function

摘要:

为解决部分亚硝化-厌氧氨氧化(PN/A)反应存在的硝酸盐残留问题,将PN/A颗粒污泥与固相反硝化(SPD)系统相耦合,用于高氨氮废水的连续处理。在逐级提高颗粒污泥自养脱氮效能的基础上,系统考察了石英砂、聚丁二酸丁二醇酯(PBS)和羟基丁酸戊酸共聚酯(PHBV)三种填料的SPD效能。结果表明,在高水力选择压下,PHBV的反硝化性能最佳,使PN/A-SPD组合工艺的总氮去除率超过了93%,远高于石英砂(83.6%)和PBS(85.8%)的水平。通过高通量测序与功能基因注释可知,固态碳源的溶出水平是影响生物膜菌群多样性的关键因素。其中,在PHBV表面占据主导的水解菌Clostridium-sensu-stricto-7Comamonadaceae等反硝化菌形成了稳定的协作关系,在有效洗脱自养脱氮功能菌的同时,显著提升了菌群参与多元素循环的功能潜力。上述发现对于系统优化PN/A-SPD组合工艺,推动其工程化应用具有重要的指导意义。

关键词: 自养生物脱氮, 颗粒污泥, 固态碳源, 反硝化, 菌群功能

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