Variation of N2O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

doi:10.11949/0438-1157.20190753

Abstract

Abstract:

At normal temperature of (20±2.0)℃, the characteristics of N₂O eimssion and extracellular polymeric substance(EPS) production were studied during simultaneous nitrification and denitrification(SND) process under different C/N ratio (C/N=3.0,5.0, 8.0 and 10.0). When C/N increased from 3.0 to 10.0, the heterotrophic bacteria increased rapidly, DO decreased and the nitrification process was inhibited. The effluent NH₄⁺ increased from below 0.5 mg/L to (7.85±1.42) mg/L, and the N₂O emission decreased from (2.68±0.17) mg/L to (1.02±0.12) mg/L. When C/N was 8.0, the maximum total notrogen removal efficiency was 80.4%±3.5%. The accumulation of PHA(poly-β-hydroxyalkanoate) increased firstly and then decreased. Both nitrifier denitrification and heterotrophic denitrification resulted in N₂O production. The anoxic area located in the biofilm decreased with the decreasing of C/N ratio, which weakened the denitrification process. With the increase of C/N ratio, N₂O diffused into the anoxic region of biofilm can be effectively reduced. The microbial EPS secretion decreased from (57.6±5.6) mg / g VSS to (32.7±3.2) mg / g VSS. The TB-EPS content accounts for 65.8%—68.8% of the total EPS. Under low C/N, the content of polysaccharide (PS) in compact EPS (TB-EPS) increased, the biofilm was more compact, the resistance of N₂O diffusion into the anoxic zone increased, and the release increased.

Key words: C/N ratio, simultaneous nitrification and denitrification, N₂O, extracellular polymeric substance

摘要：

在(20±2.0)℃条件下，利用序批式生物膜反应器（sequencing batch biofilm reactor，SBBR），考察不同碳氮比（C/N=3.0、5.0、8.0和10.0）下同步脱氮（simultaneous nitrification and denitrification，SND）过程N₂O释放及胞外聚合物（extracellular polymeric substance，EPS）变化。C/N由3.0增至10.0，异养菌大量增殖，曝气阶段DO降低，系统硝化性能受到抑制，SBBR系统出水NH₄⁺由0.5 mg/L以下增至（7.85±1.42）mg/L，N₂O产量由（2.68±0.17）mg/L降至（1.02±0.12）mg/L。C/N=8.0，TN去除率最大为80.4%±3.5%。反应初期，微生物体内聚β-羟基烷酸酯（PHA）增加，可为后续反硝化过程提供电子供体。AOB好氧反硝化和低氧条件下异养菌反硝化过程均可导致N₂O产生。C/N降低，SBBR内部缺氧区域减少，N₂O还原过程减弱，释放量增加；C/N增加，N₂O扩散进入生物膜内缺氧区域，促进其减量。C/N由3.0增至10.0，微生物EPS分泌由（57.6±5.6）mg / g VSS降至（32.7±3.2）mg / g VSS，其中，TB-EPS含量占65.8%~68.8%。低C/N下，紧密型EPS（TB-EPS）中多糖（PS）含量增加，生物膜更加密实，N₂O扩散进入缺氧区阻力增加，释放量增加。

关键词: C/N, 同步硝化反硝化, N₂O, 胞外多聚物

CLC Number:

X 703.1

Youkui GONG, Qiang ZHAO, Yongzhen PENG. Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio[J]. CIESC Journal, 2019, 70(12): 4847-4855.

巩有奎, 赵强, 彭永臻. 不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化[J]. 化工学报, 2019, 70(12): 4847-4855.

Figures/Tables 6

Fig.1 Schematic diagram of SBBR system

Fig.2 Performance of nitrogen removal in SBBR under different C/N ratio during the whole process

Fig.3 Effect of C/N ratio of influent on specific oxygen uptake rate (SOUR) in SBBR

Fig.4 Variation of COD, nitrogen and PHA concentrations in typical operational cycles of SBBR under different C/N ratio

Fig.5 Effect of C/N ratio of influent on total amount of EPS, LB-EPS and TB-EPS in SBBR

Fig.6 Effect of C/N ratio of influent on PN and PS amount in TB-EPS (a) and LB-EPS (b)

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Variation of N₂O emission and EPS production during simultaneous nitrification and denitrification in SBBR under different C/N ratio

不同C/N下SBBR脱氮过程N₂O释放及胞外多聚物变化

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