Startup and stability of A2N2 double sludge system denitrifying phosphorus removal process

doi:10.11949/j.issn.0438-1157.20160107

Abstract

Abstract:

The A₂N₂-SBR (anaerobic/nitrification/anoxic/nitrification) double sludge system was investigated by using low C/N domestic sewage. This study focused on the characteristics of nitrifying bacteria biofilm membrane and denitrifying phosphorus accumulating bacteria (DPAOs) domestication and cultivation, A₂N₂ double sludge denitrifying phosphorus removal process denitrification and phosphorus removal characteristics of the startup process. The results showed that by connecting sludge to A²/O-SBR unit and N-SBR unit, respectively to culture domestication phosphorus accumulating bacteria sludge and nitrifying bacteria biofilms separately. By using the A²/O-SBR unit effluent as the influent of N-SBR nitrifying units, the nitrification biofilm was grown up successfully within 25 d. The ammonia nitrogen removal rate was stable above 93%. In the A²/O-SBR unit, the sludge mixture was operated under anaerobic/aerobic (A/O) condition firstly. Then, the activated sludge was conducted under anaerobic/anoxic (A/A) condition, which reinforced the effect of the inoculating sludge denitrifying phosphorus removal commendably. After 43 d, the denitrifying phosphorus accumulating bacteria (DPAOs) was successfully enriched. DPAOs accounted PAOs for 67.81%. The denitrifying phosphorus removal rate steadily stayed above 77.9%. After startup successfully, about 73%, 13% COD of raw water was used by phosphorus accumulating bacteria during the A²/O-SBR unit anaerobic phase and was removed in the N-SBR unit aeration process, respectively. The A₂N₂ system effluent COD, NH⁺₄-N, PO₄³^--P, TN concentrations was 40.6, 0, 0.4 and 13.5 mg·L^-1, respectively, meeting national level A emission standards. It was suggested that A₂N₂ double sludge system denitrifying phosphorus removal process with good denitrification and phosphorus removal performance.

Key words: A₂N₂ double sludge system, denitrifying phosphorus removal, domestic sewage, sequencing batch reactor, startup

摘要：

采用低C/N比实际生活污水，以A₂N₂-SBR（厌氧/硝化/缺氧/硝化）双污泥系统为研究对象，重点考察了A₂N₂系统启动过程中的脱氮除磷特性。试验结果表明：采用在A²/O-SBR和N-SBR单元分别接种种泥，分开培养驯化聚磷菌污泥和硝化菌生物膜，并利用A²/O-SBR单元的出水作为N-SBR单元的进水，25 d好氧硝化菌生物膜挂膜成功，氨氮去除率稳定在93%以上；A²/O-SBR单元采用先厌氧/好氧（A/O）后厌氧/缺氧（A/A）的运行方式，43 d成功培养富集了反硝化聚磷菌（DPAOs），DPAOs占PAOs的67.81%，反硝化除磷率在77.9%以上；启动成功后原水中约73%和13%的COD分别在A²/O-SBR单元的厌氧段和N-SBR单元曝气过程中被去除，系统出水COD、NH⁺₄-N、PO₄³^--P、TN浓度分别为40.6、0、0.4、13.5 mg·L^-1，达到国家《城镇污水处理厂污染物排放标准》（GB 18918—2002）一级A排放标准。

关键词: A₂N₂双污泥系统, 反硝化除磷, 生活污水, 序批式反应器, 启动

CLC Number:

X703.1

WANG Meixiang, ZHAO Weihua, WANG Shuying, ZHANG Yong, PENG Yongzhen, PAN Cong, HUANG Yu. Startup and stability of A₂N₂ double sludge system denitrifying phosphorus removal process[J]. CIESC Journal, 2016, 67(7): 2987-2997.

王梅香, 赵伟华, 王淑莹, 张勇, 彭永臻, 潘聪, 黄宇. A₂N₂双污泥系统反硝化除磷工艺的启动与稳定[J]. 化工学报, 2016, 67(7): 2987-2997.

References 27

[1]	HAMADA K, KUBA T, TORRICO V, et al. Comparison of nutrient removal efficiency between pre- and post-denitrification wastewater treatments[J]. Water Science and Technology, 2006, 53(9): 169-175.
[2]	KUBA T, VANLOOSDRECHT M, HEIJNEN J J. Phosphorus and nitrogen removal with minimal COD requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system[J]. Water Research, 1996, 30(7): 1702-1710.
[3]	SMOLDERS G, VANLOOSDRECHT M, HEIJNEN J J. A metabolic model for the biological phosphorus removal process[J]. Water Science and Technology, 1995, 31(2): 79-93.
[4]	邱立平, 孙成江, 王嘉斌, 等. 反硝化除磷技术综述[J]. 济南大学学报(自然科学版), 2015, (3): 161-166. QIU L P, SUN C J, WANG J B, et al. Research advances of denitrifying phosphorous removal technology[J]. Journal of University of Jinan (Science and Technology), 2015, (3): 161-166.
[5]	KUBA T, VANLOOSDRECHT M, BRANDSE F A, et al. Occurrence of denitrifying phosphorus removing bacteria in modified UCT-type wastewater treatment plants[J]. Water Research, 1997, 31(4): 777-786.
[6]	KUBA T, SMOLDERS G, VANLOOSDRECHT M, et al. Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor[J]. Water Science and Technology, 1993, 27(5/6): 241-252.
[7]	罗固源, 罗宁, 吉芳英, 等. 新型双泥生物反硝化除磷脱氮工艺[J]. 中国给水排水, 2002, (9): 4-7. LUO G Y, LUO N, JI F Y, et al. New biological denitrification process with two-sludge for nitrogen and phosphorus removal [J]. China Water & Wastewater, 2002, (9): 4-7.
[8]	刘莹, 彭永臻, 王淑莹. A2N工艺的固有弊端分析及其对策研究[J]. 工业用水与废水, 2010, (6): 1-5. LIU Y, PENG Y Z, WANG S Y. A study on inherent shortcomings of A2N process and countermeasures[J]. Industrial Water & Wastewater, 2010, (6): 1-5.
[9]	ZHOU Y, PIJUAN M, YUAN Z. Development of a 2-sludge, 3-stage system for nitrogen and phosphorous removal from nutrient-rich wastewater using granular sludge and biofilms[J]. Water Research, 2008, 42(12): 3207-3217.
[10]	王建华, 陈永志, 彭永臻. A2O-BAF工艺处理低C/N值生活污水的快速启动[J]. 中国给水排水, 2010, (15): 32-35. WANG J H, CHEN Y Z, PENG Y Z. Fast start-up of A2O /BAF system for treatment of low C /N ratio domestic sewage [J]. China Water & Wastewater, 2010, (15): 32-35.
[11]	杨庆娟, 王淑莹, 刘莹, 等. A2N双污泥反硝化除磷系统微生物的先间歇后连续培养及快速启动[J]. 环境科学, 2008, (8): 2249-2253. YANG Q J, WANG S Y, LIU Y, et al. First sequencing then connecting cultivation of the organisms and fast start-up of A2N two-sludge system [J]. Environmental Science, 2008, (8): 2249-2253.
[12]	张勇, 王淑莹, 赵伟华, 等. 中试规模AAO-曝气生物滤池双污泥系统的启动运行[J]. 化工学报, 2015, 66(10): 4228-4235. ZHANG Y, WANG S Y, ZHAO W H, et al. Start-up of pilot-scale AAO-BAF two-sludge system [J]. CIESC Journal, 2015, 66(10): 4228-4235.
[13]	张淼, 彭永臻, 王聪, 等. 三段式硝化型生物接触氧化反应器的启动及特性[J]. 中国环境科学, 2015, 35(1): 101-109. ZHANG M, PENG Y Z, WANG C, et al. The start-up and characterization of a three-stage nitrification biological contact oxidation reactor[J]. China Environmental Science, 2015, 35(1): 101-109.
[14]	ZHANG W, PENG Y, REN N, et al. Improvement of nutrient removal by optimizing the volume ratio of anoxic to aerobic zone in AAO-BAF system[J]. Chemosphere, 2013, 93(11): 2859-2863.
[15]	王建华, 陈永志, 彭永臻. 硝化型曝气生物滤池的挂膜与启动[J]. 环境工程学报, 2010, (10): 2199-2203. WANG J H, CHEN Y Z, PENG Y Z. Biofilm formation and startup of nitrification biological aeration filter[J]. Acta Scientiae Circumstantiae, 2010(10): 2199-2203.
[16]	王亚宜, 彭永臻, 潘绵立, 等. 生物除磷系统聚糖菌的代谢机理及菌群结构[J]. 哈尔滨工业大学学报, 2008, (8): 1319-1324. WANG Y Y, PENG Y Z, PAN M L, et al. The metabolic mechanism of biological phosphorus removal system glycogen-accumulating organisms and flora structure[J]. Journal of Harbin Institute of Technology, 2008, (8): 1319-1324.
[17]	OEHMEN A, ZENG R J, KELLER J, et al. Modeling the aerobic metabolism of polyphosphate-accumulating organisms enriched with propionate as a carbon source[J]. Water Environment Research, 2007, 79(13): 2477-2486.
[18]	VAN LOOSDRECHT M, SMOLDERS G J, KUBA T, et al. Metabolism of micro-organisms responsible for enhanced biological phosphorus removal from wastewater - use of dynamic enrichment cultures[J]. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 1997, 71(1/2): 109-116.
[19]	SAITO T, BRDJANOVIC D, VAN LOOSDRECHT M. Effect of nitrite on phosphate uptake by phosphate accumulating organisms[J]. Water Research, 2004, 38(17): 3760-3768.
[20]	张为堂, 薛晓飞, 庞洪涛, 等. 碳氮比对AAO-BAF工艺运行性能的影响[J]. 化工学报, 2015, 66(5): 1925-1930. ZHANG W T, XUE X F, PANG H T, et al. Effect of C/N on performance of AAO-BAF process[J]. CIESC Journal, 2015, 66(5): 1925-1930.
[21]	张为堂, 侯锋, 刘青松, 等. AA-BAF连续流反硝化除磷系统的运行性能[J]. 中南大学学报(自然科学版), 2014, (11): 4087-4092. ZHANG W T, HOU F, LIU Q S, et al. Performance of a continuous denitrifying phosphorous removal system: anaerobic/anoxic process integrated with BAF(AA-BAF) [J]. Journal of Central South University(Science and Technology), 2014, (11): 4087-4092.
[22]	张为堂, 薛同来, 彭永臻, 等. AAO-BAF反硝化除磷系统的二次启动特性[J]. 化工学报, 2014, 65(2): 658-663. ZHANG W T, XUE T L, PENG Y Z, et al. Performance of denitrifying phosphorus removal system (AAO-BAF) during secondary start-up[J]. CIESC Journal, 2014, 65(2): 658-663.
[23]	王春英. 反硝化除磷工艺中二次曝气作用分析[J]. 江苏环境科技, 2008, (4): 72-74. WANG C Y. Analysis of secondary aeration's effect on denitrifying dephosphatation process[J]. Jiangsu Environmental Science and Technology, 2008, (4): 72-74.
[24]	WACHTMEISTER A, KUBA T, VAN LOOSDRECHT M, et al. A sludge characterization assay for aerobic and denitrifying phosphorus removing sludge[J]. Water Research, 1997, 31(3): 471-478.
[25]	THIRD K A, BURNETT N, CORD-RUWISCH R. Simultaneous nitrification and denitrification using stored substrate (PHB) as the electron donor in an SBR[J]. Biotechnology and Bioengineering, 2003, 83(6): 706-720.
[26]	张为堂, 侯锋, 刘青松, 等. HRT和曝气量对AAO-BAF系统反硝化除磷性能的影响[J]. 化工学报, 2014, 65(4): 1436-1442. ZHANG W T, HOU F, LIU Q S, et al. Effect of HRT and aeration rate on performance of AAO-BAF system for denitrifying phosphorus removal. [J]. CIESC Journal, 2014, 65(4): 1436-1442.
[27]	陈永志, 彭永臻, 王建华, 等. A2/O-曝气生物滤池工艺处理低C/N比生活污水脱氮除磷[J]. 环境科学学报, 2010, (10): 1957-1963. CHEN Y Z, PENG Y Z, WANG J H, et al. Biological phosphorus and nitrogen removal in low C/N ratio domestic sewage treatment by a A2/O-BAF combined system[J]. Acta Scientiae Circumstantiae, 2010, (10): 1957-1963.

Startup and stability of A₂N₂ double sludge system denitrifying phosphorus removal process

A₂N₂双污泥系统反硝化除磷工艺的启动与稳定

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 27

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Yingying TAN, Xiaoqing LIU, Lin WANG, Lisheng HUANG, Xiuzhen LI, Zhanwei WANG. Experimental study on startup dynamic characteristics of R1150/R600a auto-cascade refrigeration cycle [J]. CIESC Journal, 2023, 74(S1): 213-222.
[2]	Gang WANG,Yaxuan XIONG,Yuting WU,Peng XU,Guanghui LENG,Chongfang MA. Startup and isothermal performance of high-temperature molten salt heat pipe [J]. CIESC Journal, 2020, 71(11): 5099-5106.
[3]	SUN Yawen, ZHANG Jianhua, PENG Yongzhen, WANG Shuying. Effect of additional carbon sources on nitrogen and phosphorus removal characteristics in A²/O-BCO process [J]. CIESC Journal, 2018, 69(8): 3626-3634.
[4]	GAO Mengjia, WANG Shuying, WANG Shanyun, PENG Yongzhen. Impact of domestic sewage on mature anammox granular sludge [J]. CIESC Journal, 2017, 68(5): 2066-2073.
[5]	WANG Meng, BIAN Wei, HOU Aiyue, KAN Ruizhe, WANG Wenxiao, ZHANG Shuyan, LI Jun. Characteristics of partial nitrification and denitrification via two-stage aeration [J]. CIESC Journal, 2016, 67(4): 1497-1504.
[6]	WANG Meixiang, ZHAO Weihua, HUANG Yu, PAN Cong, PENG Yongzhen, WANG Shuying. Effect of nitrification duration distribution ratio in N-SBR unit on performance of A₂N₂ system operation [J]. CIESC Journal, 2016, 67(12): 5259-5267.
[7]	ZHAO Mengyue, WANG Bo, GUO Yuanyuan, PENG Yongzhen. Efficient organics utilization of low C/N ratio domestic sewage through partial nitritation SBR [J]. CIESC Journal, 2016, 67(11): 4825-4836.
[8]	ZHANG Weitang, XUE Xiaofei, PANG Hongtao, ZHANG Jie, LI Dong, PENG Yongzhen. Effect of C/N on performance of AAO-BAF process [J]. CIESC Journal, 2015, 66(5): 1925-1930.
[9]	WANG Cong, WANG Shuying, ZHANG Miao, PENG Yongzhen, ZENG Wei. Analysis of COD, N and P in denitrifying phosphorus removal under multivariate condition [J]. CIESC Journal, 2015, 66(4): 1467-1475.
[10]	LUO Xionglin, ZUO Ruixiang, FENG Aixiang, XU Feng. Modified switch control with buffer heating stage in chemical process startup [J]. CIESC Journal, 2015, 66(2): 647-654.
[11]	ZHANG Jianhua, PENG Yongzhen, ZHANG Miao, WANG Shuying, WANG Cong. Effect of different electron acceptor ratios on removal of nitrogen and phosphorus and conversion and utilization of internal carbon source [J]. CIESC Journal, 2015, 66(12): 5045-5053.
[12]	ZHANG Yong, WANG Shuying, ZHAO Weihua, SUN Shihao, PENG Yongzhen, ZENG Wei. Start-up of pilot-scale AAO-BAF two-sludge system [J]. CIESC Journal, 2015, 66(10): 4228-4235.
[13]	SONG Guang, ZHAO Yongchen, QIU Tong, LU Guangming, ZHAO Jinsong, CHEN Bingzhen. Recent progress in energy saving and emission reduction technologies in startup and shutdown processes of ethylene plants [J]. CIESC Journal, 2014, 65(7): 2696-2703.
[14]	LUO Yahong, LI Dong, BAO Linlin, XU Da, CAI Yan'an, ZHANG Jie. Shortcut nitrification and denitrifying phosphorus removal in improved A²/O technique with long SRT [J]. CIESC Journal, 2014, 65(12): 4985-4996.
[15]	YAN Lilong1，HAO Guoxin1，LIU Yu1，WEN Yan1，WU Zujing1，CHEN Yiyang1，ZHANG Yingmin2. Analysis of removal efficiency of SBR on rural sewage with rice husk as carrier [J]. Chemical Industry and Engineering Progree, 2014, 33(09): 2484-2488.