Characteristics of partial nitrification and denitrification via two-stage aeration

doi:10.11949/j.issn.0438-1157.20151266

Abstract

Abstract:

At normal temperature of 20—23℃, the domestic wastewater was treated in a sequencing batch reactor (SBR). The specific growth rate of ammonia oxidizing bacteria (AOB) was kept higher than nitrite oxidizing bacteria (NOB) via the two-stage aeration. After 80 cycles, the rapid startup of the partial nitrification was realized and operated steadily. The results showed that the specific oxygen uptake rate (SOUR) could reflect the variation of the specific growth rate of AOB when chemical oxygen demand (COD) had been largely degraded. Analyzing the scanning electron microscopy (SEM) images in different periods, it indicated that the quantity of bacillus was decreased and the quantity of spherical or elliptic spherical bacteria was increased. The establishment of 16S rDNA clone library indicated that the species of bacteria were rich and the microbes belonged to eight different groups of bacteria. Moreover, the β-Proteobacteria and Bacteroidetes were the dominant bacterial groups.

Key words: partial nitrification, aeration, sequencing batch reactor, domestic wastewater, specific oxygen uptake rate, molecular biology

摘要：

在常温(20~23℃)条件下,采用SBR反应器处理生活污水,通过两段式曝气的方法,控制氨氧化细菌(AOB)的比增长速率高于亚硝酸盐氧化细菌(NOB),经过80个周期的运行实现了短程硝化的快速启动并稳定运行。当COD降解过程结束后,比耗氧速率(SOUR)可以直观反映AOB比增长速率的变化规律。短程硝化实现前后的扫描电镜(SEM)图片表明,在短程硝化实现过程中,污泥中的杆状菌逐渐减少,球状和椭球状细菌逐渐增加。对克隆文库的结果进行分析,发现污泥中的细菌分属8个不同的细菌类群,种类比较丰富,其中优势菌群为β-变形菌和拟杆菌门。

关键词: 短程硝化, 曝气, SBR反应器, 生活污水, 比耗氧速率, 分子生物学

CLC Number:

X703.5

WANG Meng, BIAN Wei, HOU Aiyue, KAN Ruizhe, WANG Wenxiao, ZHANG Shuyan, LI Jun. Characteristics of partial nitrification and denitrification via two-stage aeration[J]. CIESC Journal, 2016, 67(4): 1497-1504.

王盟, 卞伟, 侯爱月, 阚睿哲, 王文啸, 张舒燕, 李军. 两段式曝气工艺的短程硝化反硝化特性[J]. 化工学报, 2016, 67(4): 1497-1504.

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