Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater

doi:10.11949/j.issn.0438-1157.20180219

Abstract

Abstract:

To study how the incubated sulfate reducing bacteria (SRB) sediment affects U(Ⅵ) bioreduction, the sediment together with ethanol was added into the sediment-groundwater microcosms, which were compared with microcosms amended with both pure SRB and ethanol and with ethanol only. The results indicated that uranium concentrations of the microcosms amended with the incubated SRB sediment together with ethanol, the pure SRB together with ethanol, and ethanol decreased below 0.05 mg·L^-1 (GB 23727-2009) at day 19, 22 and 28, respectively. The diversity of microbial communities capable of reducing U(Ⅵ) in all the microcosms had no significant difference. The abundances of microbial communities capable of reducing U(Ⅵ) in the microcosms amended with the incubated SRB sediment together with ethanol, and the pure SRB together with ethanol, in which ethanol were 35.3%, 32.5% and 13.1%, respectively. The abundance of microbial communities capable of reducing U(Ⅵ) increased when the incubated SRB sediment together with ethanol was added into the sediment-groundwater microcosm. It is indicated that incubated SRB sediment could accelerate the bioreduction of U(Ⅵ) in the microcosm.

Key words: U(Ⅵ), waste water, sediment, reduction, remediation

摘要：

通过添加富集得到的含有丰富的硫酸盐还原菌的沉积物到沉积物-地下水微模型中，并添加乙醇作为碳源，分别与接种硫酸盐还原菌并添加碳源组和仅添加碳源组相比较，研究富集的硫酸盐还原菌沉积物对地下水中U（Ⅵ）还原的作用。结果表明，添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组中的铀浓度分别在第19、22和28 d下降至GB 23727-2009规定的0.05 mg·L^-1排放标准以下。各组沉积物中具有还原U（Ⅵ）功能的微生物群落的多样性差异较小。添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组的沉积物中具有还原U（Ⅵ）功能的微生物群落的丰度分别为35.3%、32.5%和13.1%。由此可见，富集的硫酸盐还原菌沉积物增加了微模型中具有还原U（Ⅵ）功能的微生物群落的丰度，从而促进了U（Ⅵ）的还原。

关键词: U(Ⅵ), 废水, 沉积物, 还原, 修复

CLC Number:

LI Dianxin, HU Nan, HUANG Chao, DING Dexin, LI Guangyue, WANG Yongdong. Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater[J]. CIESC Journal, 2018, 69(8): 3619-3625.

李殿鑫, 胡南, 黄超, 丁德馨, 李广悦, 王永东. 富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究[J]. 化工学报, 2018, 69(8): 3619-3625.

References 33

[1]	DING D X, LI S M, HU N, et al. Bioreduction of U(Ⅵ) in groundwater under anoxic conditions from a decommissioned in situ leaching uranium mine[J]. Bioprocess & Biosystems Engineering, 2015, 38(4):661-669.
[2]	ZHANG H, CHENG M, LIU W, et al. Characterization of uranium in the extracellular polymeric substances of anaerobic granular sludge used to treat uranium-contaminated groundwater[J]. RSC Advances, 2017, 7(85):54188-54195.
[3]	CAMACHO L M, DENG S, PARRA R R. Uranium removal from groundwater by natural clinoptilolite zeolite:effects of pH and initial feed concentration[J]. Journal of Hazardous Materials, 2010, 175(1/2/3):393-398.
[4]	DING D, XU F, HU N, et al. Uranium speciation in sediments in microcosms before and after incubation under anoxic conditions[J]. Journal of Radioanalytical and Nuclear Chemistry, 2015, 303(1):925-933.
[5]	TANG G, WATSON D B, WU W, et al. U(Ⅵ) bioreduction with emulsified vegetable oil as the electron donor-model application to a field test[J]. Environmental Science & Technology, 2013, 47(7):3218-3225.
[6]	LUO W, WU W, YAN T, et al. Influence of bicarbonate, sulfate, and electron donors on biological reduction of uranium and microbial community composition[J]. Applied Microbiology and Biotechnology, 2007, 77(3):713-721.
[7]	GIHRING T M, ZHANG G, BRANDT C C, et al. A limited microbial consortium is responsible for extended bioreduction of uranium in a contaminated aquifer[J]. Applied and Environmental Microbiology, 2011, 77(17):5955-5965.
[8]	LUO J, WEBER F, CIRPKA O A, et al. Modeling in-situ uranium(Ⅵ) bioreduction by sulfate-reducing bacteria[J]. Journal of Contaminant Hydrology, 2007, 92(1):129-148.
[9]	SANI R K, PEYTON B M, AMONETTE J E, et al. Reduction of uranium(Ⅵ) under sulfate-reducing conditions in the presence of Fe(Ⅲ)-(hydr)oxides[J]. Geochimica et Cosmochimica Acta, 2004, 68(12):2639-2648.
[10]	LI D, HU N, DING D, et al. An experimental study on the inhibitory effect of high concentration bicarbonate on the reduction of U(Ⅵ) in groundwater by functionalized indigenous microbial communities[J]. Journal of Radioanalytical and Nuclear Chemistry, 2016, 307(2):1011-1019.
[11]	ZHOU C. The versatile roles of sulfate-reducing bacteria for uranium bioremediation[D]. Arizona State University, 2014.
[12]	ZHANG P, HE Z, NOSTRAND J D V, et al. Dynamic succession of groundwater sulfate-reducing communities during prolonged reduction of uranium in a contaminated aquifer[J]. Environmental Science & Technology, 2017, 51(7):3609-3620.
[13]	SHENG L, FEIN J B. Uranium reduction by Shewanella oneidensis MR-1 as a function of NaHCO3 concentration:surface complexation control of reduction kinetics[J]. Environmental Science & Technology, 2014, 48(7):3768-3775.
[14]	GILSON E R, HUANG S, JAFFÉ P R. Biological reduction of uranium coupled with oxidation of ammonium by Acidimicrobiaceae bacterium A6 under iron reducing conditions[J]. Biodegradation, 2015, 26(6):475-482.
[15]	SHELOBOLINA E S, VRIONIS H A, FINDLAY R H, et al. Geobacter uraniireducens sp. nov., isolated from subsurface sediment undergoing uranium bioremediation[J]. International Journal of Systematic and Evolutionary Microbiology, 2008, 58(Pt 5):1075-1078.
[16]	SHENG L, SZYMANOWSKI J, FEIN J B. The effects of uranium speciation on the rate of U(Ⅵ) reduction by Shewanella oneidensis MR-1[J]. Geochimica et Cosmochimica Acta, 2011, 75(12):3558-3567.
[17]	KAVITHA S, SELVAKUMAR R, SATHISHKUMAR M, et al. Nitrate removal using Brevundimonas diminuta MTCC 8486 from ground water[J]. Water Science & Technology. A Journal of the International Association on Water Pollution Research, 2009, 60(2):517-524.
[18]	SENKO J M, ZHANG G, MCDONOUGH J T, et al. Metal reduction at low pH by a Desulfosporosinus species:implications for the biological treatment of acidic mine drainage[J]. Geomicrobiology Journal, 2009, 26(2):71-82.
[19]	HWANG C, WU W, GENTRY T J, et al. Bacterial community succession during in situ uranium bioremediation:spatial similarities along controlled flow paths[J]. ISME Journal, 2008, 3(1):47-64.
[20]	SANI R K, PEYTON B M, DOHNALKOVA A. Toxic effects of uranium on Desulfovibrio desulfuricans G20[J]. Environmental Toxicology and Chemistry, 2006, 25(5):1231-1238.
[21]	刘晓波, 董少刚, 刘白薇, 等. 内蒙古土默川平原地下水水文地球化学特征及其成因[J]. 地球学报, 2017, 38(6):919-929. LIU X B, DONG S G, LIU B W, et al. Hydrogeoehemical characteristics and genesis of groundwater in the Tumochuan plain of Inner Mongolia[J]. Acta Geoscientica Sinica, 2017, 38(6):919-929.
[22]	LEIGH M B, WU W, CARDENAS E, et al. Microbial communities biostimulated by ethanol during uranium (Ⅵ) bioremediation in contaminated sediment as shown by stable isotope probing[J]. Frontiers of Environmental Science & Engineering, 2015, 9(3):453-464.
[23]	MOON H S, MCGUINNESS L, KUKKADAPU R K, et al. Microbial reduction of uranium under iron-and sulfate-reducing conditions:effect of amended goethite on microbial community composition and dynamics[J]. Water Research, 2010, 44(14):4015-4028.
[24]	SAKAGUCHI S, KANO K, IKEDA T. Effect of pH on the hydrogenase activity of Desulfovibrio vulgaris[J]. Electroanalysis, 2004, 16(13/14):1166-1171.
[25]	KAVITHA S, SELVAKUMAR R, SATHISHKUMAR M, et al. Nitrate removal using Brevundimonas diminuta MTCC 8486 from ground water[J]. Water Science and Technology, 2009, 60(2):517-524.
[26]	SU W, ZHANG L, LI D, et al. Dissimilatory nitrate reduction by Pseudomonas alcaliphila with an electrode as the sole electron donor[J]. Biotechnology & Bioengineering, 2012, 109(11):2904-2910.
[27]	LÜCKER S, WAGNER M, MAIXNER F, et al. A Nitrospira metagenome illuminates the physiology and evolution of globally important nitrite-oxidizing bacteria[J]. Proceedings of the National Academy of Sciences, 2010, 107(30):13479-13484.
[28]	FLETCHER K E, BOYANOV M I, THOMAS S H, et al. U(Ⅵ) reduction to mononuclear U(Ⅳ) by Desulfitobacterium Species[J]. Environmental Science & Technology, 2010, 44(12):4705-4709.
[29]	O'LOUGHLIN E J, KELLY S D, COOK R E, et al. Reduction of uranium(Ⅵ) by mixed iron(Ⅱ)/iron(Ⅲ) hydroxide (green rust):formation of UO2 nanoparticles[J]. Environmental Science & Technology, 2003, 37(4):721-727.
[30]	JUNIER P, SUVOROVA E I, BERNIERLATMANI R. Effect of competing electron acceptors on the reduction of U(Ⅵ) by Desulfotomaculum reducens[J]. Geomicrobiology Journal, 2010, 27(5):435-443.
[31]	PRAKASH O, GIHRING T M, DALTON D D, et al. Geobacter daltonii sp. nov., an Fe(Ⅲ)-and uranium(Ⅵ)-reducing bacterium isolated from a shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination[J]. International Journal of Systematic and Evolutionary Microbiology, 2010, 60(3):546-553.
[32]	ANDERSON R T, VRIONIS H A, ORTIZBERNAD I, et al. Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer[J]. Appl. Environ. Microbiol., 2003, 69(10):5884-5891.
[33]	BRILEYA K A, CAMILLERI L B, ZANE G M, et al. Biofilm growth mode promotes maximum carrying capacity and community stability during product inhibition syntrophy[J]. Frontiers in Microbiology, 2014, 5(693):1-13.