CIESC Journal ›› 2023, Vol. 74 ›› Issue (6): 2647-2654.DOI: 10.11949/0438-1157.20230271

• Energy and environmental engineering • Previous Articles     Next Articles

Application of quinone profile method to indicate structure and activity of functional microbial community in trichloroethylene-contaminated soil

Lixiang ZHU1(), Moye LUO1, Xiaodong ZHANG2, Tao LONG2, Ran YU1()   

  1. 1.College of Energy and Environment, Southeast University, Nanjing 211189, Jiangsu, China
    2.Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing 210042, Jiangsu, China
  • Received:2023-03-21 Revised:2023-05-17 Online:2023-07-27 Published:2023-06-05
  • Contact: Ran YU

醌指纹法指示三氯乙烯污染土功能微生物活性应用研究

朱理想1(), 罗默也1, 张晓东2, 龙涛2, 余冉1()   

  1. 1.东南大学能源与环境学院,江苏 南京 211189
    2.生态环境部南京环境科学研究所,江苏 南京 210042
  • 通讯作者: 余冉
  • 作者简介:朱理想(2000—),男,硕士研究生,2636861418@qq.com
  • 基金资助:
    国家自然科学基金项目(52270119);国家重点研发计划项目(2018YFC1803100)

Abstract:

This study aimed to evaluate the potential of bioremediation of organic polluted sites using quinone profile method to analyze the activity of functional microbial in trichloroethylene (TCE)-contaminated soil. Microcosm experiments were conducted to investigate the degradation process of TCE in soil and to create a quinone profile. The profile was compared with existing quinone spectral libraries, and a quinone with potential indicator microbial activity for TCE-contaminated soil was selected. By analyzing the changes of the total quinone concentration (TQ) and the concentration of the selected quinone, changes in the active microbial population and functional microbial activity during the degradation process were observed and validated as feasible indicators for reflecting potential microbial activity. The results indicated that primary degradation occurred during 0—50 days, with a degradation rate of TCE over 65% for all three soil groups. Differences in the biodegradation pathways of TCE were observed in the three soil groups. UQ-8, UQ-9, MK-6, and MK-8 were identified as characteristic quinones in TCE-contaminated soil. There were significant positive correlations between TQ, microbial biomass carbon (MTOC), total concentration of characteristic quinones and dehydrogenase activity (P<0.05). Therefore, the quinone profile method can provide an important monitoring method guidance for the monitoring of natural attenuation risk control or the implementation of bioremediation in organically polluted sites.

Key words: organic compounds, contamination, remediation, quinone profile method, microbial activity

摘要:

基于对有机污染场地生物修复潜能评价需求,通过微宇宙试验探讨了醌指纹法解析三氯乙烯(TCE)污染土壤功能微生物活性的潜力。为此,探讨了土壤TCE的降解过程,绘制了土壤醌指纹图谱,通过与现有醌的谱库对比解析了醌指纹图谱并筛选出TCE污染土壤具有指示功能菌群活性潜能特征醌。根据总醌浓度(TQ)和特征醌浓度变化,解析了降解过程中活性微生物量变化和功能菌群活性变化并验证了其可分别作为反映活性微生物量和功能微生物活性潜在指标的可行性。结果表明,主要降解期间在0~50 d, TCE的降解率均在65%以上,三组土壤TCE生物降解途径存在差异;UQ-8、UQ-9、MK-6、MK-8为TCE污染土壤中的特征醌;TQ和微生物量碳(MTOC)、特征醌总浓度与脱氢酶活性均存在显著正相关(P<0.05)。因此,醌指纹法可为有机污染场地监测自然衰减风险管控或生物修复的实施提供重要的监测方法指导。

关键词: 有机化合物, 污染, 修复, 醌指纹法, 微生物活性

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