PRODUCTION OF THROMBOLYTIC ENZYME BY DO-STAT BATCH FERMENTATION EMPLOYING Bacillus subtilis Hl-986

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PRODUCTION OF THROMBOLYTIC ENZYME BY DO-STAT BATCH FERMENTATION EMPLOYING Bacillus subtilis Hl-986

Han Runlin, Zhang Xiaoyong and Li Zuohu, Zhang Jianan (Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080) (Dept. Of Chem.Eng., Tsinghua Vniv.,Beijing 100084)

Online:2000-12-30 Published:2000-12-30

枯草杆菌溶栓酶的恒溶氧发酵研究

韩润林,张小勇,李佐虎,张建安

中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,中国科学院化工冶金研究所生化工程国家重点实验室!北京 100080,清华大学化学工程系!北京100084

Abstract

Abstract: DO-stat batch fermentation of a thrombolytic enzyme from Bacillus subtilis HL-986 was studied. The thrombolytic enzyme yield reached 930IU/ml by DO-stat fermentation, and was increased 38.8% compared with constant aeration rate. The optimum DO-stat level is 15% controlled in the beginning of fermentstion.

摘要： 本文以枯草杆菌（Ｂａｃｉｌｌｕｓ　ｓｕｂｔｉｌｉｓ）ＨＬ－９８６为试验菌株，对其恒溶氧发酵生产溶栓酶的的过程进行了研究，并与恒通气速率发酵作了比较。结果表明：与恒通气速率发酵相比，恒溶氧发酵可使溶栓酶生产能力提高３８．８％，在２２ｈ时溶栓酶活力可达９３０ＩＵ／ｍｌ，最佳条件是在发酵初期控制恒溶氧１５％。

CLC Number:

TQ92

Han Runlin, Zhang Xiaoyong and Li Zuohu, Zhang Jianan (Inst. Chem. Metall., Chinese Academy of Sciences, Beijing 100080) (Dept. Of Chem.Eng., Tsinghua Vniv.,Beijing 100084). PRODUCTION OF THROMBOLYTIC ENZYME BY DO-STAT BATCH FERMENTATION EMPLOYING Bacillus subtilis Hl-986[J]. .

韩润林,张小勇,李佐虎,张建安. 枯草杆菌溶栓酶的恒溶氧发酵研究 [J]. CIESC Journal.

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