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Effects of Hydrophobicity of Ethylene Oxide-Propylene Oxide Copolymers on Phase Diagrams of
Aqueous Two-Phase Systems and Partition Behaviors of Cephalexin and 7-
Aminodeacetoxicephalos Doranic Acid

ZHU Jianhang; WEI Dongzhi; CAO Xuejun   

  1. State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China
    University of Science and Technology, Shanghai 200237, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-04-28 Published:2002-04-28
  • Contact: ZHU Jianhang

高矣物的特性对双水相相图和头孢氨苄及7-ADCA分配行为的影响

朱建航; 魏东芝; 曹学君   

  1. State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China
    University of Science and Technology, Shanghai 200237, China
  • 通讯作者: 朱建航

Abstract: A series of ethylene oxide (EO)-propylene oxide (PO) random co-polymers (EOPO) were used to
formaqueous two-phase systems (ATPS) with ammonium sulfate. Effects of EOPO’s properties on
the phase separationbehaviors and on the partition of cephalexin and 7-
aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass
and molar ratio of EO to PO of EOPO could greatly influence partition behaviorsof
cephalexin and 7-ADCA as well as the binodal curve of ATPS. With the increase of molar mass
of co-polymeror the decrease of molar ratio of EO to PO, the critical point of ATPS
decreased, the binodal curve became moreasymmetry, and both cephalexin and 7-ADCA followed
the same tendency to partition into the polymer-poorbottom phase. The experimental results
shows that it is feasible to partition cephalexin and 7-ADCA in either thepolymer-rich top
phase or the polymer-poor bottom phase by choosing a specific phase-forming EOPO.

Key words: ethylene oxide (EO) and propylene oxide (PO) random co-polymer (EOPO), aqueous two-phase sys-telns, partition coefficient, phase diagram, cephalexin

摘要: A series of ethylene oxide (EO)-propylene oxide (PO) random co-polymers (EOPO) were used to
formaqueous two-phase systems (ATPS) with ammonium sulfate. Effects of EOPO’s properties on
the phase separationbehaviors and on the partition of cephalexin and 7-
aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass
and molar ratio of EO to PO of EOPO could greatly influence partition behaviorsof
cephalexin and 7-ADCA as well as the binodal curve of ATPS. With the increase of molar mass
of co-polymeror the decrease of molar ratio of EO to PO, the critical point of ATPS
decreased, the binodal curve became moreasymmetry, and both cephalexin and 7-ADCA followed
the same tendency to partition into the polymer-poorbottom phase. The experimental results
shows that it is feasible to partition cephalexin and 7-ADCA in either thepolymer-rich top
phase or the polymer-poor bottom phase by choosing a specific phase-forming EOPO.

关键词: ethylene oxide (EO) and propylene oxide (PO) random co-polymer (EOPO);aqueous two-phase sys-telns;partition coefficient;phase diagram;cephalexin