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Improved Production of Paclitaxel from Suspension Culture of Taxus chinensis var. mairei by
in situ Extraction with Organic Solvents

WEI Zuojun; YUAN Yingjin; WU Zhaoliang; WU Jinchuan   

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology,
    Tianjin University,Tianjin 300072, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-04-28 Published:2003-04-28
  • Contact: WEI Zuojun

有机溶剂原位萃取法提高悬浮培养中国红豆杉细胞紫杉醇产量

未作君; 元英进; 吴兆亮; 吴金川   

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology,
    Tianjin University,Tianjin 300072, China
  • 通讯作者: 未作君

Abstract: The production of paclitaxel from suspension culture of Taxus chinensis var. maireiwas
improved by in situ extraction with organic solvents to avoid feedback repression and
product degradation. Oleic acid and dibutyl phthalate were proved to be suitable solvents.
The optimal volumetric percentage of organic solvents in the culture medium was found to be
around 8%, and the favorable time for their introduction was at the exponential phase of
cell growth. Paclitaxel production with the in situ extraction was ca 3-fold of that
without extraction.

Key words: extraction, organic solvent, paclitaxel, Taxus chinensis var. mairei, two-liquid-phase culture

摘要: The production of paclitaxel from suspension culture of Taxus chinensis var. maireiwas
improved by in situ extraction with organic solvents to avoid feedback repression and
product degradation. Oleic acid and dibutyl phthalate were proved to be suitable solvents.
The optimal volumetric percentage of organic solvents in the culture medium was found to be
around 8%, and the favorable time for their introduction was at the exponential phase of
cell growth. Paclitaxel production with the in situ extraction was ca 3-fold of that
without extraction.

关键词: extraction;organic solvent;paclitaxel;Taxus chinensis var. mairei;two-liquid-phase culture