Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol

Abstract

Abstract: Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase(GPDH) which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179gL－1 with initial glycerol concentration of 80gL－1 in medium, compared with 41gL－1 in the control experiment. These results were successfully reproduced for fed-batch process in an air-lift reactor.

Key words: Candida krusei, glycerol, osmotolerant yeast, osmoregulator, air-lift reactor

摘要： Candida krusei was osmotolerant yeast for the production of glycerol. Extracellular osmotic pressure was one of the key factors to induce the enzyme activity of glycerol-3-phosphate dehydrogenase(GPDH) which severely affected the glycerol productivity. Three osmoregulators such as NaCl, PEG4000 and glycerol were used to investigate their effects on yeast growth, glucose consumption and glycerol production. In order to determine the effect of extracellular glycerol concentration, different amounts of glycerol were initially supplemented as an osmoregulator to increase glycerol production. The maximum glycerol concentration attained 179gL－1 with initial glycerol concentration of 80gL－1 in medium, compared with 41gL－1 in the control experiment. These results were successfully reproduced for fed-batch process in an air-lift reactor.

关键词: Candida krusei;glycerol;osmotolerant yeast;osmoregulator;air-lift reactor

CHENGuo, YAOShanjing, GUANYixin. Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol[J]. .

陈国, 姚善泾, 关怡新. 渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响[J]. CIESC Journal.

References

1    Wang, Z.X., Zhuge, J., Fang, H.Y., Prior, B.A., “Glycerol production by microbial fermentation: A review”, Bio-technol. Adv., 19, 201—223(2001).
2    Vijaikishore, P., Karanth, N.G., “Glycerol production by immobilized cells of Pichia farinose”, Biotechnol. Lett., 8, 257—260(1986).
3    Liu, H.J., Liu, D.H., Zhong, J.J., “Novel fermentation strategy for enhancing glycerol production by Candida krusei”, Biotechnol. Prog., 19, 1615—1619(2003).
4    Remize, F., Barnavon, L., Dequin, S., “Glycerol export and glycerol-3-phosphate dehydrogenase, but not glyc-erol phosphatase, are rate limiting for glycerol produc-tion in Saccharomyces cerevisiae”, Metab. Eng., 3,     301—312(2001).
5    Liu, Y.Q., Liu, D.H., Su, Q., Xie, D.M., “Glycerol pro-duction by Candida krusei employing NaCl as an osmo-regulator in batch and continuous fermentations”, Bio-technol. Lett., 24, 1137—1140(2002).
6    Liu, Y.Q., Liu, D.H., Su, Q., “Glycerol production by batch and continuous fermentation with NaCl as osmo-regulator”, J. Chem. Ind. Eng. (China)., 54, 259—263(2003). (in Chinese)
7    Blomberg, A., Adler, L., “Physiology of osmotolerance in fungi”, Adv. Microbiol. Physiol., 33, 145—212(1992).
8    Miller, G.L., “Use of dinitrosalicylic acid reagent for de-termination of reducing sugars”, Anal. Chem., 31, 426—428(1959).
9    Ashwath, M.F.R., Newman, A.A., Analytical Methods for Glycerol, Academic Press, New York, 19—21(1979).
10    Kalle, G.P., Naik, S.C., “Continuous fed-batch vacuum fermentation system for glycerol from molasses by sul-fite process”, J. Ferment. Technol., 63, 411—414(1985).
11    Schade, A.L., Farber, E., “Glycerol”, U. S. Pat., 2414838(1947).
12    Liu, H.J., Liu, D.H., Zhong, J.J., “Oxygen limitation improves glycerol production by Candida krusei in a bioreactor”, Process Biochem., 39, 1899—1902(2004).
13    Liu, Y.Q., Liu, D.H., Su, Q., Liu, J.R., Xie, D.M., “Criti-cal influence of osmotic pressure on continuous produc-tion of glycerol by an osmophilic strain of Candida krusei in a multistage cascade bioreactor”, Process Bio-chem., 38, 427—432(2002).
14    Blomberg, A., “Metabolic surprises in Saccharomyces cerevisiae during adaption to saline conditions: Question, some answers and a model”, FEMS Microbial. Lett., 182, 86—97(2000).
15    Tamas, M.J., Luyten, K., Sutherland, F.C.W., Hernandez, A., Albertyn, J., Valadi, H., Prior, B.A., Kilian, S.G., Ramos, J., Gustafsson, L., Thevelein, J.M., Hohmann, S., “Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation”, Mol. Microbiol., 31,1087—1104(1999).
16    Hohmann, S., Bill, R.M., Kayingo, G., Prior, B.A., “Micro-bial MIP channels”, Trends Microbiol., 8, 33—38(2000).

[1]	Duotao PAN, Xudong WANG, Hongyan SHI, Zhilong XIU. Steady-state analysis and feedback control of continuous fermentation for bio-based 1, 3-propanediol [J]. CIESC Journal, 2022, 73(5): 2094-2100.
[2]	Mengyu LI, Dongxiang WANG, Xiaoyang ZHENG, Guizhuan XU, Chaojun DU, Chun CHANG. Preparation and adsorption properties of crude glycerol bio-based polyurethane material [J]. CIESC Journal, 2022, 73(5): 2270-2278.
[3]	Jiaren ZHANG, Haichao LIU. Phase equilibrium of transesterification reaction system between soybean oil and methanol [J]. CIESC Journal, 2022, 73(5): 1920-1929.
[4]	Hui SHANG, Lu LIU, Hanmo WANG, Wenhui ZHANG. Effect of microwave field on hydrogen bonds in glycerol aqueous solution system [J]. CIESC Journal, 2019, 70(S1): 23-27.
[5]	LEI Jiaqi, DUAN Xuezhi, QIAN Gang, ZHOU Xinggui. Effects of carbon support on glycerol oxidation over Pt-C composite catalysts in base-free conditions [J]. CIESC Journal, 2017, 68(2): 679-686.
[6]	LENG Li, ZHANG Hong, REN Xin, ZHOU Jinghong, SUI Zhijun, ZHOU Xinggui. Effect of silica chemistry on structure of Ir-Re bimetallic catalysts and catalysis for glycerol hydrogenolysis [J]. CIESC Journal, 2016, 67(2): 540-548.
[7]	WANG Song, ZHANG Zhengyan, CHANG Qianqian, LI Sanxi, ZHANG Linnan. Synthesis of glycerol monolaurate using functionalized ionic liquids catalysts [J]. CIESC Journal, 2015, 66(S1): 209-215.
[8]	ZHENG Liping, YUAN Zhenle, XIA Shuixin, CHEN Ping, HOU Zhaoyin. Super solid-base supported Cu catalysts for hydrogenolysis of glycerol to 1,2-propanediol [J]. CIESC Journal, 2015, 66(8): 3014-3021.
[9]	WANG Shizhen, YAN Zhengping, QIU Longhui, FANG Baishan. Metabolic evolution of Lactobacillus pentosus for lactic acid production from raw glycerol [J]. CIESC Journal, 2015, 66(8): 3195-3203.
[10]	ZHOU Wei, ZHAO Yujun, MA Xinbin. Continuous hydrogenolysis of glycerol to 1,2-propanediol on highly active Cu/SiO₂ catalysts [J]. CIESC Journal, 2015, 66(8): 2999-3006.
[11]	GUO Yong1，ZHANG Yongguang1，LIN Jianmin1，WANG Yanqin2. Progress in hydrogen production by the aqueous-phase reforming of glycerol [J]. Chemical Industry and Engineering Progree, 2014, 33(01): 50-55.
[12]	ZENG Hong, FANG Baishan, WANG Paifen, ZHANG Tingting. Process simulation design and techno-economic analysis of production technology of glycerol dehydrogenase [J]. CIESC Journal, 2013, 64(6): 2169-2176.
[13]	DONG Chaoqi, GENG Yanlou, AN Hualiang, ZHAO Xinqiang, WANG Yanji. Etherification of glycerol with methanol catalyzed by SO₃H-functioned ionic liquids [J]. CIESC Journal, 2013, 64(6): 2086-2091.
[14]	GAO Yixia, YAN Libin, XIN Zhong. Component confirmation and process of biodiesel produced in supercritical conditions with addition of tiny KOH [J]. CIESC Journal, 2013, 64(2): 683-688.
[15]	WANG Feng，YAN Hua，ZHANG Zhen，GUAN Changfeng，YANG Weimin. Studies on mixing performances of the servo dynamic mixer [J]. Chemical Industry and Engineering Progree, 2013, 32(11): 2574-2578.

Influence of Osmoregulators on Osmotolerant Yeast Candida krusei for the Production of Glycerol

渗透压调节剂对耐高渗菌Candida Krusei生产甘油的影响

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments