Abstract: Magnetic iron oxide（Fe3O4，γ-Fe2O3 etc.） nanoparticles have attracted researchers in various fields such as biochemical engineering and medicine industrical due to their small size，superparamagnetism and low toxicity. Magnetic chitosan microspheres are in sphere shape with a rather smooth surface. In recent years，magnetic chitosan microspheres were prepared with a mean size range of 10 nm to 2.5×105 nm. Magnetic chitosan microspheres have been preliminarily applied in various fields，including biomedicine，food engineering and wastewater treatment，etc，especially in the fields of wastewater treatment and enzyme immobilization. The preparation methods of magnetic iron oxide nanoparticles and magnetic chitosan microspheres，the recent achievements including modification methods and applications of magnetic chitosan microspheres，are reviewed in this paper.

Key words: magnetic chitosan microspheres, immobilization, Fe₃O₄, nano-material

摘要： 磁性氧化铁纳米粒子（Fe₃O₄，γ-Fe₂O₃等）因具有尺寸小、超顺磁性和低毒性等特点，已经引起了生物化工、医药工业研究领域的广泛关注。磁性壳聚糖微球具有表面非常光滑的球形结构。近年来，已经制备出了平均粒径在 10～2.5×10⁵ nm 之间的磁性壳聚糖微球，并在生物医药、食品工程和污水处理等许多领域已经取得了初步的应用，特别是在污水处理和酶固定化领域。本文综述了近年来磁性氧化铁纳米粒子和磁性壳聚糖微球的制备方法、磁性壳聚糖微球的改性方法及应用的最新研究成果。

关键词: 磁性壳聚糖微球, 固定化, 四氧化三铁, 纳米材料