Abstract: Quantum dots（QDs） have substantial advantages over organic dyes，including high stability against photobleaching，tunable colors by changing particle size，a single wavelength for simultaneous excitation of different-sized QDs and narrow emission peaks. Replacing the organic fluorophores of the conventional molecular beacon with QDs will overcome its many shortcomings. Quantum dot-molecular beacon is not only sensitive in measurement and stable against photo degradation，but also can be applied for multi-detection. In this paper，the development of quantum dot-molecular beacon in recent years is introduced. The types of MBs，linkage strategies，fluorescence quenchers and the characterization of quantum dot-molecular beacon were summarized. Moreover，its applications on nucleic-acid diagnostics are discussed，including multiple SNP detection，real-time monitoring on the living cells.

Key words: quantum dots, molecular beacon, nucleic acid detection, preparation

摘要： 量子点具有耐光漂白、颜色可调、一元激发/多元发射以及发射光谱窄等特性，故将其应用在分子信标中，必将克服传统有机染料的很多缺点。量子点分子信标不但灵敏度高、光稳定性好，而且可以进行多重检测。本文就近几年来量子点分子信标的发展进行了综述，其中针对量子点的制备系统阐述了可用于制备分子信标的量子点类型、量子点与分子信标连接的方式、量子点分子信标猝灭剂的选择及其表征。同时还详细介绍了量子点分子信标在多重SNP测定、细胞内病毒表达等核酸检测中的应用。

关键词: 量子点, 分子信标, 核酸检测, 制备