Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast

doi:10.3969/j.issn.0438-1157.2013.10.033

CIESC Journal ›› 2013, Vol. 64 ›› Issue (10): 3725-3731.DOI: 10.3969/j.issn.0438-1157.2013.10.033

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Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast

WANG Liang, XUE Chuang, BAI Fengwu

School of Life Sciences and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, China

Received:2013-03-13 Revised:2013-05-12 Online:2013-10-05 Published:2013-10-05
Supported by:
supported by the National Natural Science Foundation of China(21276038),the International S&T Cooperation Program of China(2013DFA60470),the Open Research Fund from Biofuels Key Laboratory of Chinese Academy of Sciences in Qingdao Institute of Bioenergy and Bioprocess Technology(CASKLB201303),the Fundamental Research Funds for the Central Universities(DUT11RC(3)77)and the China Postdoctoral Science Foundation(2013M530904).

气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为

王亮, 薛闯, 白凤武

大连理工大学生命科学与技术学院, 辽宁大连 116023

通讯作者: 薛闯,白凤武
作者简介:王亮(1986-),男,博士研究生。
基金资助:
国家自然科学基金项目(21276038);国家国际科技合作专项项目(2013DFA60470);中国科学院生物燃料重点实验室开放基金项目(CASKLB201303);中央高校基本科研业务费专项资金项目(DUT11RC(3)77);中国博士后科学基金面上项目(2013M530904)

Abstract

Abstract: Process oscillation is the main challenge in continuous very high gravity(VHG)ethanol fermentation,which could result in oscillation and decrease of ethanol concentration.In the present study,during continuous VHG ethanol fermentation by free cell Saccharomyces cerevisiae 4126 and self-flocculating yeast BHL01,sustained process oscillations of biomass,residual glucose,ethanol and glycerol,were observed with a period of 130—145 h.Furthermore,in the self-flocculating yeast system,ethanol concentration and productivity were higher than those in the free yeast system.VHG oscillation was significantly attenuated and then a quasi-steady state was developed instead,when gas stripping with fermentative off-gas was used to separate ethanol during continuous VHG ethanol fermentation by self- flocculating yeast.Finally,the residual glucose kept below 0.1 g·L^-1,and ethanol concentration and productivity were 110.87 g·L^-1 and 2.99 g·L^-1·h^-1,respectively.The present study provides a new strategy for the attenuation of oscillation in continuous VHG ethanol fermentation.

Key words: self-flocculating yeast, very high gravity ethanol fermentation, oscillation, gas stripping

摘要： 超高浓度(very high gravity,VHG)连续乙醇发酵过程中的振荡行为会导致发酵终点的乙醇浓度振荡和降低,是VHG连续乙醇发酵面临的主要问题。研究表明,游离酵母Saccharomyces cerevisiae 4126和自絮凝酵母BHL01在VHG连续乙醇发酵过程中,生物量、残糖、乙醇、甘油等发酵参数都呈现130～145 h的周期性振荡,且絮凝酵母发酵体系的平均乙醇浓度和乙醇生产强度都明显高于游离酵母体系。在絮凝酵母VHG连续乙醇发酵过程中利用发酵尾气气提分离乙醇,酵母细胞振荡行为被明显弱化,达到拟稳态状态,并且使发酵液中的残糖浓度控制在0.1 g·L^-1以下,平均乙醇浓度为110.87 g·L^-1,乙醇产率达到2.99 g·L^-1·h^-1。因此,本研究为弱化VHG连续乙醇发酵中的参数振荡行为提供了新的技术手段。

关键词: 自絮凝酵母, 超高浓度乙醇发酵, 振荡, 气提

CLC Number:

Q939.9

WANG Liang, XUE Chuang, BAI Fengwu. Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast[J]. CIESC Journal, 2013, 64(10): 3725-3731.

王亮, 薛闯, 白凤武. 气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为[J]. 化工学报, 2013, 64(10): 3725-3731.

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Oscillation attenuation by gas stripping in continuous very high gravity ethanol fermentation by self-flocculating yeast

气提法弱化超高浓度乙醇发酵过程中自絮凝酵母振荡行为

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