Abstract: By means of Ostwald ripening self-assembly process, Ag nanoparticles substrates of various densities and sizes were fabricated.In the investigation of surface enhanced Raman scattering (SERS)and surface enhancement fluorescence (SEF)using crystal violet (CV)as a probe molecule, it was found that Raman and luminescence intensity varied in parallel with the Ag nanoparticles size.With increasing time of self-assembly process, the changes of intensity trended to be stabilized.The results indicated that SERS and SEF could be optimized by Ostwald ripening self-assembly.The results obtained could provide reference when developing long-term stability and cost-effective bio-chemical sensors based on SERS and SEF.

Key words: surface enhanced Raman scattering;surface enhancement fluorescence, Ostwald ripening, Ag nanoparticles, self-assembly, stability

摘要： 利用Ostwald ripening自组装工艺对拉曼增强衬底进行处理，得到粒径和密度不同的银纳米颗粒拉曼增强衬底。用龙胆紫生物大分子作为探针，对其表面增强拉曼散射（SERS）和表面增强荧光（SEF）进行研究发现，拉曼和荧光光谱强度存在着相似的变化趋势，且随着自组装工艺时间的递增，强度的变化趋于稳定。研究结果表明：Ostwald ripening自组装工艺可以优化SERS和SEF增强效果及其稳定性，故其可为研制具有长期稳定性、低成本的基于SERS和SEF效应生物化学传感器件提供研究基础。

关键词: 表面增强拉曼散射, 表面增强荧光, Ostwald ripening, 银纳米颗粒, 自组装, 稳定性