Mesoporous TiO2 with different selectivities of protein immobilization performance

doi:10.3969/j.issn.0438-1157.2014.05.027

CIESC Journal

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Mesoporous TiO₂ with different selectivities of protein immobilization performance

DONG Yihui¹, AN Rong², ZHUANG Wei³, YAO Zhong⁴, ZHU Yudan¹, LIU Chang¹, LU Xiaohua¹

1 State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 210009, Jiangsu, China;
2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
3 School of Biological and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, Jiangsu, China;
4 School of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, Jiangsu, China

Received:2014-01-02 Revised:2014-03-10 Online:2014-05-05 Published:2014-05-05
Supported by:
supported by the National Natural Science Foundation of China(21136004), the National Basic Research Program of China(2013CB733501)and the Project of Priority Academic Program Development of Jiangsu High Education Institutions(PAPD).

介孔TiO₂对不同蛋白的选择性固定化性能

董依慧¹, 安蓉², 庄伟³, 姚忠⁴, 朱育丹¹, 刘畅¹, 陆小华¹

1 南京工业大学材料化学工程国家重点实验室, 江苏南京 210009;
2 Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA;
3 南京工业大学生物与制药学院, 江苏南京 211816;
4 南京工业大学食品与轻工学院, 江苏南京 210009

通讯作者: 刘畅
基金资助:
国家自然科学基金项目（21136004）；国家重点基础研究发展计划项目（2013CB733501）；江苏高校优势学科建设工程项目。

Abstract

Abstract: Mesoporous TiO₂ with adjustable pore diameter, high specific surface area and excellent biocompatibility has a good application prospect in biological molecules immobilization carriers. Mesoporous TiO₂-500 were prepared as the stationary phase packing in High Performance Liquid Chromatograph by calcinating H₂Ti₂O₅ at 500℃, and the TiO₂ samples were characterized by X-ray diffraction (XRD), N₂ adsorption-desorption and field-emmission scanning electron microscopy (FESEM). The residence time under different pH of bovine serum albumin, myoglobin and lysozyme through the new chromatograph column were tested. Nearly all three kinds of proteins were immobilized at pH =4.7, and the order of residence time of three proteins was lysozyme >myoglobin > bovine serum albumin under the condition of pH=7.4 and 11. Adsorption of three kinds of proteins on the surface of the mesoporous TiO₂-500 under atmospheric pressure was consistent with chromatographic results, and the order of the immobilization capacity of mesoporous TiO₂-500 for three kinds of proteins was lysozyme > myoglobin >bovine serum albumin. Therefore, due to the significant difference in protein immobilization performance, the mesoporous TiO₂has a potential application in protein adsorption and separation, immobilizing enzyme and other fields.

Key words: mesoporous TiO₂, protein, chromatograph, residence time, immobilization

摘要： 通过500℃下焙烧H₂Ti₂O₅制备介孔TiO₂-500作为高效液相色谱的固定相填料，测试牛血清蛋白（BSA）、肌红蛋白（myoglobin）、溶菌酶（lysozyme）在不同pH环境下的停留时间分布，结果表明，在pH=4.7，三种蛋白质几乎都固定化，在pH=7.4和11条件下，三种蛋白通过色谱柱的停留时间为溶菌酶>肌红蛋白>牛血清蛋白。三种蛋白在介孔TiO₂-500表面的宏观常压吸附量的结果与色谱结果相一致，介孔TiO₂-500对于三种蛋白的固定化能力为溶菌酶>肌红蛋白>牛血清蛋白。因此，该介孔TiO₂对不同蛋白的显著的固定化性能的差异使其在蛋白质的吸附和分离、固定化酶等领域有着潜在的应用。

关键词: 介孔TiO₂, 蛋白质, 色谱, 停留时间, 固定化

CLC Number:

DONG Yihui, AN Rong, ZHUANG Wei, YAO Zhong, ZHU Yudan, LIU Chang, LU Xiaohua. Mesoporous TiO₂ with different selectivities of protein immobilization performance[J]. CIESC Journal, DOI: 10.3969/j.issn.0438-1157.2014.05.027.

董依慧, 安蓉, 庄伟, 姚忠, 朱育丹, 刘畅, 陆小华. 介孔TiO₂对不同蛋白的选择性固定化性能[J]. 化工学报, DOI: 10.3969/j.issn.0438-1157.2014.05.027.

References 30

[1]	Katiyar A, Ji L, Smirniotis P, Pinto N G. Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size [J]. Journal of Chromatography A, 2005, 1069: 119-126
[2]	Magner E. Immobilisation of enzymes on mesoporous silicate materials [J]. Chemical Society Reviews, 2013, 42: 6213-6222
[3]	Proteins at Interfaces II [M]. American Chemical Society, 1995
[4]	Bayne L, Ulijn R V, Halling P J. Effect of pore size on the performance of immobilised enzymes [J]. Chemical Society Reviews, 2013, 42: 9000-9010
[5]	Lue Y, Lu G, Wang Y, Guo Y, Guo Y, Zhang Z, Wang Y, Liu X. Functionalization of cubic Ia3d mesoporous silica for immobilization of penicillin G acylase [J]. Advanced Functional Materials, 2007, 17: 2160-2166
[6]	Lin Z E, Yang G Y. Chemistry of porous materials: from microporous inorganic - Solids to metal-organic frameworks [J]. Chinese Journal of Structural Chemistry, 2004, 23: 1388-1398
[7]	Diaz J F, Balkus K J. Enzyme immobilization in MCM-41 molecular sieve [J]. Journal of Molecular Catalysis B-Enzymatic, 1996, 2: 115-126
[8]	Takimoto A, Shiomi T, Ino K, Tsunoda T, Kawai A, Mizukami F, Sakaguchi K. Encapsulation of cellulase with mesoporous silica (SBA-15) [J]. Microporous and Mesoporous Materials, 2008, 116: 601-606
[9]	Wang A, Liu M, Wang H, Zhou C, Du Z, Zhu S, Shen S, Ouyang P. Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation [J]. Journal of Bioscience and Bioengineering, 2008, 106: 286-291
[10]	Hartmann M. Ordered mesoporous materials for bioadsorption and biocatalysis [J]. Chemistry of Materials, 2005, 17: 4577-4593
[11]	Lee C H, Lin T S, Mou C Y. Mesoporous materials for encapsulating enzymes [J]. Nano Today, 2009, 4: 165-179
[12]	Si P, Ding S, Yuan J, Lou X W, Kim D H. Hierarchically structured one-dimensional TiO2 for protein immobilization, direct electrochemistry, and mediator-free glucose sensing [J]. ACS Nano, 2011, 5: 7617-7626
[13]	Yu J, Zhao H, Ye L, Yang H, Ku S, Yang N, Xiao N. Effect of surface functionality of magnetic silica nanoparticles on the cellular uptake by glioma cells in vitro [J]. Journal of Materials Chemistry, 2009, 19: 1265-1270
[14]	Tao Z, Toms B B, Goodisman J, Asefa T. Mesoporosity and functional group dependent endocytosis and cytotoxicity of silica nanomaterials [J]. Chemical Research in Toxicology, 2009, 22: 1869-1880
[15]	Yu J H, Ju H X. Preparation of porous titania sol-gel matrix for immobilization of horseradish peroxidase by a vapor deposition method [J]. Analytical Chemistry, 2002, 74: 3579-3583
[16]	Calleja G, Serrano D P, Sanz R, Pizarro P, Garcia A. Study on the synthesis of high-surface-area mesoporous TiO2 in the presence of nonionic surfactants [J]. Industrial & Engineering Chemistry Research, 2004, 43: 2485-2492
[17]	Liu W, Wang J G, Li W, Guo X, Lu L, Lu X, Feng X, Liu C, Yang Z. A shortcut for evaluating activities of TiO2 facets: water dissociative chemisorption on TiO2-B (100) and (001) [J]. Physical Chemistry Chemical Physics, 2010, 12: 8721-8727
[18]	Li Q, Wang X, Lu X, Tian H, Jiang H, Lü G, Guo D, Wu C, Chen B. The incorporation of daunorubicin in cancer cells through the use of titanium dioxide whiskers [J]. Biomaterials, 2009, 30: 4708-4715
[19]	Qin Danhua (秦丹华), Yao Zhong (姚忠), Wang Haoqi (王浩琦), Xiao Yifan(肖易凡), Yang Jing (杨敬), Xu Hong (徐虹), Wei Ping(韦萍). Immobilization of GGT on meso-TiO2:preparation and properties [J]. CIESC Journal (化工学报), 2011, 62(2): 378-385
[20]	Chaudhuri A S, Bose S. Isolation of protein from dilute solution by chromatographic method [J]. International Journal of Pharmacy and Engineering(IJPE),2013,1(1):12-16
[21]	Wu Z, Zhao D. Ordered mesoporous materials as adsorbents [J]. Chemical Communications, 2011, 47: 3332-3338
[22]	Wang F, Min Y, Geng X. Fast separations of intact proteins by liquid chromatography [J]. Journal of Separation Science, 2012, 35: 3033-3045
[23]	Fujimoto C. Titanium dioxide coated surfaces for capillary electrophoresis and capillary electrochromatography [J]. Electrophoresis, 2002, 23: 2929-2937
[24]	Ghazi I, Segura D, Gómez A, Fernández-Arrojo L, Alcalde M, Yates M, Rojas-Cervantes M L, Plou F J, Ballesteros A. Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the synthesis of fructo-oligosaccharides [J]. Journal of Molecular Catalysis B: Enzymatic, 2005, 35: 19-27
[25]	Hernández R, Mendieta Wejebe J, Vazquez Alcantara J, Miranda Ruvalcaba R, Garc?a Serrano L, Trujillo Ferrara J. Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios [J]. Microporous and Mesoporous Materials, 2005, 80: 25-31
[26]	Bao N Z, Shen L M, Feng X, Lu X H. High quality and yield in potassium titanate whiskers synthesized by calcination from hydrous titania [J]. Journal of the American Ceramic Society, 2004, 87: 326-330
[27]	He M, Feng X, Lu X H, Ji X Y, Liu C, Bao N Z, Xie J W. Application of an ion-exchange model to the synthesis of fibrous titanate derivatives [J]. Journal of Chemical Engineering of Japan, 2003, 36: 1259-1262
[28]	Lin Huaxiang (林华香), Wang Xuxu (王绪绪), Fu Xianzhi (付贤智). Properties and distribution of the surface hydroxyl groups of TiO2 [J]. Progress in Chemistry(化学进展), 2007, 19: 665-670
[29]	Wang D, Zhao J, Chen B, Zhu C. Lattice vibration fundamentals in nanocrystalline anatase investigated with Raman scattering [J]. Journal of Physics-Condensed Matter, 2008, 20(18): 085212
[30]	Yang Zhuhong(杨祝红), Wang Yanfang (王艳芳), Li Licheng (李力成), Wang Changsong (王昌松), Lu Xiaohua(陆小华). Pore structure modification of mesoporous TiO2 for immobilization of protein [J]. Journal of Nanjing University of Technology(南京工业大学学报), 2012, 34: 7-17

Mesoporous TiO₂ with different selectivities of protein immobilization performance

介孔TiO₂对不同蛋白的选择性固定化性能

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