CIESC Journal ›› 2023, Vol. 74 ›› Issue (8): 3457-3471.DOI: 10.11949/0438-1157.20230211

• Biochemical engineering and technology • Previous Articles     Next Articles

Preparation of magnetic agarose microspheres based on a two-fluid nozzle and their protein adsorption properties

Yan GAO1(), Peng WU1, Chao SHANG2, Zejun HU1, Xiaodong CHEN1()   

  1. 1.School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, Jiangsu, China
    2.Beaverbio Medical Engineering Company Limited, Suzhou 215123, Jiangsu, China
  • Received:2023-03-09 Revised:2023-06-25 Online:2023-10-18 Published:2023-08-25
  • Contact: Xiaodong CHEN


高燕1(), 伍鹏1, 尚超2, 胡泽君1, 陈晓东1()   

  1. 1.苏州大学材料与化学化工学部化工与环境工程学院,江苏 苏州 215123
    2.苏州海狸生物医学工程有限公司,江苏 苏州 215123
  • 通讯作者: 陈晓东
  • 作者简介:高燕(1998—),女,硕士研究生,


Aiming at the problems of the existing magnetic agarose microspheres (MAM) preparation technology, such as long process flow, poor repeatability, and difficulty in achieving large-scale and continuous production, a new preparation method of magnetic agarose microspheres based on two-fluid nozzle atomization technology was proposed. The variation of droplet size under different atomization conditions was investigated. Moreover, under the optimized spray conditions, MAM with high sphericity, small particle size and fast magnetic response were successfully prepared. The microspheres after sieving were prepared as DEAE anion exchanger (DEAE-MAM), and bovine serum albumin (BSA) was used as a model protein to explore the protein adsorption performance of DEAE-MAM with different particle sizes. The results show that the spray conditions affected the droplet size and thus the particle size by changing the gas-liquid ratio under the premise that the water phase properties were consistent. The DEAE-MAM with the smallest particle size (d32 = 36 μm) had the largest ion exchange capacity (192.5 μmol/ml) and the highest saturated adsorption capacity (150.0 mg/ml).

Key words: gas-liquid flow, gel, granulation, atomization, ion exchange, protein adsorption


针对现有磁性琼脂糖微球(MAM)制备技术工艺流程长、重复性差、难以实现规模化及连续化生产等问题,提出了一种基于双流体喷嘴雾化技术的新型磁性琼脂糖微球制备方法;探究了不同雾化条件下,液滴尺寸变化的规律;在优化后的喷雾条件下,成功制备出球形度高、粒径小、磁响应迅速的磁性琼脂糖微球;将筛分后的微球制备成DEAE阴离子交换剂(DEAE-MAM),并以牛血清白蛋白(BSA)为模型蛋白,探究了不同粒径下的DEAE-MAM的蛋白吸附性能。结果表明,在保证水相性质一致的前提下,喷雾条件通过改变气液比影响液滴尺寸,进而影响微球粒径;粒径最小的DEAE-MAM(d32 = 36 μm)离子交换容量最大(192.5 μmol/ml),饱和吸附量最高(150.0 mg/ml)。

关键词: 气液两相流, 凝胶, 造粒, 雾化, 离子交换, 蛋白吸附

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