CIESC Journal ›› 2018, Vol. 69 ›› Issue (8): 3619-3625.DOI: 10.11949/j.issn.0438-1157.20180219

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Experimental study on U(Ⅵ) bioreduction by incubated sulfate reducing bacteria sediment in groundwater

LI Dianxin1,2, HU Nan1,2, HUANG Chao1,2, DING Dexin1,2, LI Guangyue1,2, WANG Yongdong1,2   

  1. 1 Key Discipline Laboratory for National Defence for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, Hunan, China;
    2 Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources, University of South China, Hengyang 421001, Hunan, China
  • Received:2018-02-28 Revised:2018-04-09 Online:2018-08-05 Published:2018-08-05
  • Supported by:

    supported by the National Natural Science Foundation of China (11775106, U1401231, 51274124).

富集的硫酸盐还原菌沉积物生物还原地下水中U(Ⅵ)的实验研究

李殿鑫1,2, 胡南1,2, 黄超1,2, 丁德馨1,2, 李广悦1,2, 王永东1,2   

  1. 1 南华大学铀矿冶生物技术国防重点学科实验室, 湖南 衡阳 421001;
    2 南华大学极贫铀资源绿色开发技术湖南省重点实验室, 湖南 衡阳 421001
  • 通讯作者: 丁德馨
  • 基金资助:

    国家自然科学基金项目(11775106,U1401231,51274124)。

Abstract:

To study how the incubated sulfate reducing bacteria (SRB) sediment affects U(Ⅵ) bioreduction, the sediment together with ethanol was added into the sediment-groundwater microcosms, which were compared with microcosms amended with both pure SRB and ethanol and with ethanol only. The results indicated that uranium concentrations of the microcosms amended with the incubated SRB sediment together with ethanol, the pure SRB together with ethanol, and ethanol decreased below 0.05 mg·L-1 (GB 23727-2009) at day 19, 22 and 28, respectively. The diversity of microbial communities capable of reducing U(Ⅵ) in all the microcosms had no significant difference. The abundances of microbial communities capable of reducing U(Ⅵ) in the microcosms amended with the incubated SRB sediment together with ethanol, and the pure SRB together with ethanol, in which ethanol were 35.3%, 32.5% and 13.1%, respectively. The abundance of microbial communities capable of reducing U(Ⅵ) increased when the incubated SRB sediment together with ethanol was added into the sediment-groundwater microcosm. It is indicated that incubated SRB sediment could accelerate the bioreduction of U(Ⅵ) in the microcosm.

Key words: U(Ⅵ), waste water, sediment, reduction, remediation

摘要:

通过添加富集得到的含有丰富的硫酸盐还原菌的沉积物到沉积物-地下水微模型中,并添加乙醇作为碳源,分别与接种硫酸盐还原菌并添加碳源组和仅添加碳源组相比较,研究富集的硫酸盐还原菌沉积物对地下水中U(Ⅵ)还原的作用。结果表明,添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组中的铀浓度分别在第19、22和28 d下降至GB 23727-2009规定的0.05 mg·L-1排放标准以下。各组沉积物中具有还原U(Ⅵ)功能的微生物群落的多样性差异较小。添加硫酸盐还原菌沉积物组、接种硫酸盐还原菌组和对照组的沉积物中具有还原U(Ⅵ)功能的微生物群落的丰度分别为35.3%、32.5%和13.1%。由此可见,富集的硫酸盐还原菌沉积物增加了微模型中具有还原U(Ⅵ)功能的微生物群落的丰度,从而促进了U(Ⅵ)的还原。

关键词: U(Ⅵ), 废水, 沉积物, 还原, 修复

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