Synergistic relationship between hydrocarbon degrading and emulsifying strain SL-1 and endogenous bacteria during oil displacement

doi:10.11949/0438-1157.20220442

Abstract

Abstract:

In order to explore the synergistic oil displacement mechanism of endogenous and exogenous bacteria in the process of heavy oil recovery, the hydrocarbon-philic emulsification bacteria Geobacillus stearothermophilus SL-1 was used as the exogenous bacteria to investigate the synergistic viscosity reduction and hydrocarbon reduction performance of the bacteria and endogenous flora. The efficacy of the indigenous and exogenous bacteria to reduce crude oil viscosity and degrade hydrocarbons of heavy oil were investigated. The structural characteristics of the bacterial community were analyzed by using high-throughput sequencing of 16S rDNA, and the synergistic relationship between indigenous and exogenous bacteria was evaluated. The results showed that long-chain alkanes in heavy oil were degraded significantly and the viscosity of heavy oil decreased by approximately 79.5% under the synergistic effect of strain SL-1 and the endogenous bacteria. Hydrocarbon degrading bacteria and hydrogen producing bacteria, such as Desulfomicrobium, Rhizobium, Legionella, Coprothermobacter, and Gelria, were activated after the addition of strain SL-1, and gas production and methane content increased. The stability of the microbial community was enhanced, which improved the oil recovery performance of the functional bacteria. A species correlation analysis showed that strain SL-1 was positively correlated with Pseudothermotoga, Coprothermobacter and Gelria. The synergy between these species can promote the process of hydrocarbon degradation and methane production, which will improve the process of heavy oil recovery. This study provides theoretical support for the field application of strain SL-1.

Key words: Geobacillus, synergistic, microbial enhanced oil recovery, degradation, petroleum, biotechnology

摘要：

为了探究稠油开采过程内-外源菌的协同驱油机理，以嗜烃乳化菌Geobacillus stearothermophilus SL-1作为外源菌，考察了该菌与内源菌群的协同降黏、降烃性能。通过16S rDNA扩增子测序，探讨了内-外源菌的协同作用关系。研究结果表明，添加菌株SL-1后，稠油中的长链烷烃被显著降解，原油黏度降低约79.5%。菌群结构分析表明，菌株SL-1的加入有效激活了烃降解菌、产氢菌等采油功能菌，产气量及甲烷含量升高，同时增强了菌群结构的稳定性，进而有利于采油功能菌代谢性能的发挥。物种相关性分析表明，菌株SL-1与Pseudothermotoga、Coprothermobacter、Gelria等产氢菌呈正相关性，这些物种间的相互协同可推动烃降解及产甲烷等进程，进而有利于提高稠油的采收率。本研究为菌株SL-1在稠油开采中的现场应用提供了理论支撑。

关键词: Geobacillus, 协同, 微生物采油, 降解, 石油, 生物技术

CLC Number:

TE 357.9

Caifeng LI, Xiao WANG, Gangjian LI, Junzhang LIN, Weidong WANG, Qinglin SHU, Yanbin CAO, Meng XIAO. Synergistic relationship between hydrocarbon degrading and emulsifying strain SL-1 and endogenous bacteria during oil displacement[J]. CIESC Journal, 2022, 73(9): 4095-4102.

李彩风, 王晓, 李岗建, 林军章, 汪卫东, 束青林, 曹嫣镔, 肖盟. 嗜烃乳化菌SL-1与内源菌协同驱油的菌群作用关系研究[J]. 化工学报, 2022, 73(9): 4095-4102.

Figures/Tables 10

Table 1 Composition of each sample

编号	样品组成
A	地层水
B	地层水+菌株SL-1
C	地层水+营养激活剂
D	地层水+菌株SL-1+营养激活剂

Fig.1 Cumulative gas production under different nutrition and flora combinations

Table 2 Analysis of gas composition

序列	名称	组成/%
序列	名称	C组	D组
1	H₂	0.047	0.083
2	CO₂	74.534	48.898
3	CO	0.007	0.003
4	CH₄	24.976	50.828
5	乙烷	0.193	0.135
6	乙烯	0.009	0.005
7	丙烷	0.082	0.027
8	异丁烷	0.066	0.008
9	正丁烷	0.051	0.005
10	异戊烷	0.007	0.003
11	正戊烷	0.020	0.005
12	2, 2-二甲基丙烷	0.007	0.000

Fig.2 Changes of crude oil viscosity

Fig.3 Relative content of n-alkanes in different samples

Table 3 Alpha diversity analysis

项目	A_7	B_7	C_7	D_7
Shannon	2.3549	1.9228	1.1961	1.4179
Simpson	0.1782	0.238	0.4451	0.3236
Chao1	144.9406	80.6471	34.3903	42.8603

Fig.4 Species composition at phylum level during stimulation process

Fig.5 Clustering heatmap at genus level

Fig.6 Principal component analysis of samples during stimulation

Fig.7 Correlation analysis among species under the synergistic efficacy of indigenous and exogenous bacteria

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