苯酚对污水生物脱氮系统亚硝积累及污泥性能的影响

doi:10.3969/j.issn.0438-1157.2013.07.045

化工学报 ›› 2013, Vol. 64 ›› Issue (7): 2641-2649.DOI: 10.3969/j.issn.0438-1157.2013.07.045

苯酚对污水生物脱氮系统亚硝积累及污泥性能的影响

路聪聪, 王淑莹, 葛士建, 张琼, 杨锦辉, 彭永臻

北京工业大学北京市水质科学与水环境恢复重点实验室, 北京市污水脱氮除磷处理与过程控制工程技术研究中心, 北京 100124

收稿日期:2012-11-01 修回日期:2013-01-07 出版日期:2013-07-05 发布日期:2013-07-05
通讯作者: 王淑莹
作者简介:路聪聪(1987- ),女,硕士研究生。
基金资助:
国家高技术研究发展计划项目(2011AA060903-02);2012年学科与研究生教育-创新人才培养计划-博士生创新基金项目(YB201210)。

Effects of phenol on nitrite accumulation and sludge performance in biological nitrogen removal system

LU Congcong, WANG Shuying, GE Shijian, ZHANG Qiong, YANG Jinhui, PENG Yongzhen

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Engineering Research Center of Beijing for Wastewater Treatment, Beijing University of Technology, Beijing 100124, China

Received:2012-11-01 Revised:2013-01-07 Online:2013-07-05 Published:2013-07-05
Supported by:
supported by the High-tech Research and Development Program of China (2011AA060903-02)and the Training Plan of Innovative Talents,Project of Disciplines and Postgraduate Education,2012(YB201210).

摘要/Abstract

摘要： 采用SBR缺氧/好氧反应工艺,研究了不同苯酚浓度对脱氮过程中亚硝积累与污泥性能的影响。结果表明,苯酚浓度在0~90 mg·L^-1变化时系统出现2次明显亚硝酸盐积累,最终稳定维持在70%±5%,低浓度(0~30 mg·L^-1)系统亚硝酸盐积累恢复是微生物改变自身结构及分泌胞外聚合物导致;高浓度(60~90 mg·L^-1)苯酚条件下亚硝积累是由于苯酚对AOB(ammonia-oxidizing bacteria,氨氮氧化细菌)和NOB(nitrite-oxidizing bacteria,亚硝酸氧化细菌)抑制作用引起的微生物种群改变形成。氨氮氧化速率和氮氧化物生成速率由10.85 mg N·(g MLSS)^-1·h^-1和10.12 mg N·(g MLSS)^-1·h^-1降低至2.79 mg N·(g MLSS)^-1·h^-1和2.32 mg N·(g MLSS)^-1·h^-1,亚硝酸盐积累率和氮氧化物生成速率呈现负相关性,与苯酚浓度呈正相关;荧光原位杂交表明苯酚的抑制使得硝化菌群结构发生了变化,AOB 相对数量由2.80%增加为9.30%。苯酚的可降解性使得系统污泥浓度由2500 mg·L^-1左右上升至5870 mg·L^-1。当EPS(extracellular polymeric substances,胞外聚合物)总量由67.20 mg·(g VSS)^-1减少至32.10 mg·(g VSS)^-1时,SVI从165 ml·g^-1降到50 ml·g^-1。亚硝酸盐积累、丝状菌和胞外聚合物是引起活性污泥系统SVI变化的原因,其中NAR影响最大,丝状菌次之。

关键词: 苯酚, 亚硝积累, EPS, 硝化速率, 污泥沉降性

Abstract: In the pre-denitrification of anoxic-aerobic reaction process,nitrite accumulation and sludge performance at different phenol concentrations were investigated.The results showed that there were two obvious nitrite accumulations finally stabilized at 70%±5% when phenol varied from 0 to 90 mg·L^-1.At low phenol concentration (0 to 30 mg·L^-1),nitrite accumulation and recovery were caused by microbial structure varieties and secreting extracellular polymer.At high phenol concentration (60—90 mg·L^-1),nitrite accumulation was caused by microbial population changes of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria was caused by phenol inhibition.Ammonia oxidation rates and nitrogen oxide production rates varied from 10.85 mg N·(g MLSS)^-1·h^-1 and 10.12 mg N·(g MLSS)^-1·h^-1 to 2.79 mg N·(g MLSS)^-1·h^-1 and 2.32 mg N·(g MLSS)^-1·h^-1,respectively.Nitrite accumulation rate had a negative correlation with nitrogen oxide production rate,whereas a positive correlation to phenol concentration.Fluorescence in situ hybridization showed that nitrifying bacteria communities changed due to phenol inhibition,with AOB relative quantity increasing from 2.80% to 9.30%.As carbon source,phenol caused sludge concentration increase from 2500 mg·L^-1 up to 5870 mg·L^-1.When total extracellular polymeric substances declined from 67.20 mg·(g VSS)^-1 to 32.10 mg·(g VSS)^-1,sludge volume index (SVI)accordingly changed from 165 ml·g^-1 to 50 ml·g^-1.Nitrite accumulation,filamentous bacteria and extracellular polymer contributed to SVI changes in the activated sludge system,and nitrite accumulation was the main reason followed by filamentous bacteria.

Key words: phenol, nitrite accumulation, EPS, nitrifying rate, sludge settleability

中图分类号:

X703

路聪聪, 王淑莹, 葛士建, 张琼, 杨锦辉, 彭永臻. 苯酚对污水生物脱氮系统亚硝积累及污泥性能的影响[J]. 化工学报, 2013, 64(7): 2641-2649.

LU Congcong, WANG Shuying, GE Shijian, ZHANG Qiong, YANG Jinhui, PENG Yongzhen. Effects of phenol on nitrite accumulation and sludge performance in biological nitrogen removal system[J]. CIESC Journal, 2013, 64(7): 2641-2649.

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苯酚对污水生物脱氮系统亚硝积累及污泥性能的影响

Effects of phenol on nitrite accumulation and sludge performance in biological nitrogen removal system

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