化工学报 ›› 2013, Vol. 64 ›› Issue (11): 4181-4186.DOI: 10.3969/j.issn.0438-1157.2013.11.040

• 生物化学工程与技术 • 上一篇    下一篇

氧化还原电位控制的自絮凝酵母自动重复批次发酵

刘黎阳, 刘晨光, 白凤武   

  1. 大连理工大学生命科学与技术学院, 辽宁 大连 116024
  • 收稿日期:2013-04-21 修回日期:2013-06-25 出版日期:2013-11-05 发布日期:2013-11-05
  • 通讯作者: 刘晨光,白凤武
  • 作者简介:刘黎阳(1990-),男,硕士研究生。
  • 基金资助:

    中国博士后科学基金项目(2012M511149);中央高校基本科研业务费(DUT11RC(3)76)。

Automatic consecutive batch ethanol fermentation with self-flocculating yeast under ORP control

Liyang, LIU Chenguang, BAI Fengwu   

  1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China
  • Received:2013-04-21 Revised:2013-06-25 Online:2013-11-05 Published:2013-11-05
  • Supported by:

    supported by the China Postdoctoral Science Foundation(2012M511149)and the Fundamental Research Funds for the Central Universities of China(DUT11RC(3)76).

关键词: 生物燃料, 发酵, 生物过程, 重复批次, 氧化还原电位, 自絮凝酵母

Abstract: Consecutive batch fermentation with self-flocculating yeast realized recycling of cell and increased ethanol yield and productivity.Oxidoreduction potential(ORP)control was used in this process to automatically feed the fresh medium at the end of every batch by judging the uprising of ORP curve.When ORP value was controlled at-100 mV and initial glucose were chosen as 200 g·L-1(industrial concentration)and 250 g·L-1(very high gravity),compared with batch fermentation,accumulated biomass in consecutive batch sharply declined by 42.4%(~200 g·L-1,5 batches)and 16.3%(~250 g·L-1,6 batches),respectively.On the other hand,compared with manual consecutive batch fermentation,automatic process greatly shortened fermentation time by 17.8%(~200 g·L-1)and 15.2%(~250 g·L-1).Consequently,ethanol productivities were enhanced by 21.7% and 17.9%,respectively.

Key words: biofuel, fermentation, bioprocess, consecutive batch, ORP, self-flocculating yeast

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