关键词:

混合模式吸附层析, 免疫球蛋白, 猪血, 沉淀, 纤维蛋白原

Abstract:

Mixed-mode adsorption chromatography is a new kind of bioseparation technology，which normally combines the electrostatic and hydrophobic interactions.It has a unique property of salt-independent adsorption that can adsorb the target protein efficiently under either high-salt or low-salt condition.In the present work mixed-mode adsorption was used to separate immunoglobulin from porcine plasma.Firstly different methods were compared to precipitate the fibrinogen from porcine plasma，and the precipitation with ammonium sulfate was optimized.Then the adsorption isotherm and kinetics of mixed-mode adsorbent for immunoglobulin were investigated，and the conditions of adsorption and desorption were determined.Finally the mixed-mode adsorption chromatography was used to separate immunoglobulin directly from the supernate of ammonium sulfate precipitation.The yield was about 11.5 mg.8226ml^-1，and the purity of immunoglobulin reached about 77.4% with the image analysis of SDS-PAGE.The process developed in the present work provided a new way for the comprehensive utilization of porcine plasma resources.

Key words:

混合模式吸附层析, 免疫球蛋白, 猪血, 沉淀, 纤维蛋白原